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. 2023 Jun 1;28(11):4498.
doi: 10.3390/molecules28114498.

Preparation of Biocompatible Manganese Selenium-Based Nanoparticles with Antioxidant and Catalytic Functions

Yang Yu  1 , Peng Fan  1 , Jinfeng Li  1 , Shige Wang  1
Affiliations

Preparation of Biocompatible Manganese Selenium-Based Nanoparticles with Antioxidant and Catalytic Functions

Yang Yu et al. Molecules. .

Abstract

The specificity of the tumor microenvironment (TME) severely limits the effectiveness of tumor treatment. In this study, we prepared a composite nanoparticle of manganese dioxide and selenite by a one-step redox method, and their stability under physiological conditions was improved with a bovine serum protein modification to obtain MnO2/Se-BSA nanoparticles (SMB NPs). In the SMB NPs, manganese dioxide and selenite endowed the SMB NPs with acid-responsive and catalytic, and antioxidant properties, respectively. The weak acid response, catalytic activity, and antioxidant properties of composite nanoparticles were verified experimentally. Moreover, in an in vitro hemolysis assay, different concentrations of nanoparticles were incubated with mouse erythrocytes, and the hemolysis ratio was less than 5%. In the cell safety assay, the cell survival ratio was as high as 95.97% after the co-culture with L929 cells at different concentrations for 24 h. In addition, the good biosafety of composite nanoparticles was verified at the animal level. Thus, this study helps to design high-performance and comprehensive therapeutic reagents that are responsive to the hypoxia, weak acidity, hydrogen peroxide overexpression nature of TME and overcome the limitations of TME.

Keywords: antioxidant; catalytic; manganese dioxide; tumor microenvironment.

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Conflict of interest statement

The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

Figures

Figure 1
Figure 1
(a) Schematic illustration of the preparation of SMB NPs. (b) SEM diagram of SMB NPs. (c,d) Elemental distribution mappings of SMB NPs: (d) O, (e) Mn, and (f) Se.
Figure 2
Figure 2
(a) DLS plots of SMB NPs in saline. (b,c) XPS spectra of Mn and Se.
Figure 3
Figure 3
(a) Time-dependent UV-Vis-NIR spectra of SMB in saline. (b) Pictures of SMB in saline over time. (c) Time-dependent UV-Vis-NIR spectra of SMB in CBS. (d) Pictures of SMB in CBS over time.
Figure 4
Figure 4
(a) The color change in SMB NPs placed in different buffer solutions in the first 2 min. (b) SMB NPs catalyzed bubble production in the first 2 min since its contact with H2O2. PBS was set as control.
Figure 5
Figure 5
(a) The ABTS+ scavenging ratio of SMB NPs. (b) The color fading of ABTS+ solution after being treated with SMB NPs of different concentrations. (c) The DPPH scavenging ratio of SMB NPs. (d) The color fading of DPPH solution after being treated with SMB NPs of different concentrations.
Figure 6
Figure 6
(a) Hemolysis ratio of mRBCs treated with water, PBS, and SMB NPs with different experimental conditions. (b) Images of mRBCs corresponding to (a). (c) Survival ratio of L929 cells treated with SMB NPs with different experimental conditions. (d) Live/dead staining images of untreated, 50, 100, and 200 μg/mL SMB NPs -treated L929 cells.
Figure 7
Figure 7
(a) Body weight change curves of mice treated with PBS and SMB NPs. (b,c) Serum biochemical parameters of mice at different time points after treatment with PBS and SMB NPs.
Figure 8
Figure 8
(a–i) Plots of blood parameters of mice at different time points after treatment with PBS and SMB NPs.
See this image and copyright information in PMC

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