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. 2023 May;13(5):599-603.
doi: 10.5455/OVJ.2023.v13.i5.12. Epub 2023 May 13.

Comparative performance evaluation of blood film microscopy for the diagnosis of bovine trypanosomosis by some laboratories in North-central Nigeria

Jones Soladoye Akinbobola  1 , Solomon Oluwole Okaiyeto  2 , Anthony Kojo Sackey  3 , Lushaikyaa Allam  2 , Bisalla Mohammed  4 , Jerome Nyhalah Dinga  5   6 , Prosper C Chukwuemeka  1 , Lucas Cunningham  7
Affiliations

Comparative performance evaluation of blood film microscopy for the diagnosis of bovine trypanosomosis by some laboratories in North-central Nigeria

Jones Soladoye Akinbobola et al. Open Vet J. 2023 May.

Abstract

Background: Due to its affordability in disease-affected communities and suitability for field application, microscopy has historically been considered the gold standard for field diagnosis of trypanosomosis in rural settings.

Aim: This works aims to compare the performance of microscopists on bovine trypanosome microscopy by organizing the first comparative assessment on a correct reading of slides by laboratory professionals using the read slide results and a structured interviewer-administered questionnaire in North-central Nigeria.

Methods: Ten participants were addressed, as they were sent a panel of two slides (Slide 1: No Trypanosome present; Slide 2: Trypanosome present) and a questionnaire.

Results: All participants greater than 41 years old reported correctly the presence and absence of parasites on slides. Only 3/8 of microscopists from routine diagnostic laboratories reported correctly the presence of the parasite.

Conclusion: Our study confirmed errors in reading slides. Therefore, training of microscopists besides a nationwide quality assessment is recommended.

Keywords: Blood film microscopy; Bovine trypanosomosis; Evaluation; North-central Nigeria; Performance.

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Conflict of interest statement

The authors declare that there is no conflict of interest regarding the publication of this paper.

Figures

Fig. 1.
Fig. 1.. Flow chart demonstrating the study design.
Fig. 2.
Fig. 2.. (A) Uninfected sample. (B) Trypanosoma brucei (arrowed) infected sample.
See this image and copyright information in PMC

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