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. 2023 Oct;290(19):4744-4761.
doi: 10.1111/febs.16886. Epub 2023 Jun 20.

Syntaxin 5-dependent phosphorylation of the small heat shock protein Hsp42 and its role in protein quality control

Doryaneh Ahmadpour  1   2 , Navinder Kumar  1 , Arthur Fischbach  1 , Srishti Chawla  1 , Per O Widlund  1 , Thomas Nyström  1
Affiliations
Free article

Syntaxin 5-dependent phosphorylation of the small heat shock protein Hsp42 and its role in protein quality control

Doryaneh Ahmadpour et al. FEBS J. 2023 Oct.
Free article

Abstract

The small heat shock protein Hsp42 and the t-SNARE protein Sed5 have central roles in the sequestration of misfolded proteins into insoluble protein deposits in the yeast Saccharomyces cerevisiae. However, whether these proteins/processes interact in protein quality control (PQC) is not known. Here, we show that Sed5 and anterograde trafficking modulate phosphorylation of Hsp42 partially via the MAPK kinase Hog1. Such phosphorylation, specifically at residue S215, abrogated the co-localization of Hsp42 with the Hsp104 disaggregase, aggregate clearance, chaperone activity, and sequestration of aggregates to IPOD and mitochondria. Furthermore, we found that Hsp42 is hyperphosphorylated in old cells leading to a drastic failure in disaggregation. Old cells also displayed a retarded anterograde trafficking, which, together with slow aggregate clearance and hyperphosphorylation of Hsp42, could be counteracted by Sed5 overproduction. We hypothesize that the breakdown of proper PQC during yeast aging may, in part, be due to a retarded anterograde trafficking leading to hyperphosphorylation of Hsp42.

Keywords: aging; phosphorylation; protein quality control; small Heat shock protein Hsp42; syntaxin 5.

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References

    1. Walter S & Buchner J (2002) Molecular chaperones-cellular machines for protein folding. Angew Chem Int ed Engl 41, 1098-1113.
    1. Schneider KL, Nystrom T & Widlund PO (2018) Studying spatial protein quality control, proteopathies, and aging using different model misfolding proteins in S. cerevisiae. Front Mol Neurosci 11, 249.
    1. Ahmadpour D, Babazadeh R & Nystrom T (2020) Hitchhiking on vesicles: a way to harness age-related proteopathies? FEBS J 287, 5068-5079.
    1. Morimoto RI (2008) Proteotoxic stress and inducible chaperone networks in neurodegenerative disease and aging. Genes Dev 22, 1427-1438.
    1. Kaytor MD & Warren ST (1999) Aberrant protein deposition and neurological disease. J Biol Chem 274, 37507-37510.

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