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. 2023:2671:219-239.
doi: 10.1007/978-1-0716-3222-2_13.

Protein Cage-Stabilized Emulsions: Formulation and Characterization

Mridul Sarker #  1 , Samuel Watts #  1   2 , Stefan Salentinig  3 , Sierin Lim  4
Affiliations

Protein Cage-Stabilized Emulsions: Formulation and Characterization

Mridul Sarker et al. Methods Mol Biol. 2023.

Abstract

The formulation of Pickering emulsions using protein cages is gaining interest for applications in molecular delivery. Despite the growing interest, methods to investigate the at the liquid-liquid interface are limited. This chapter describes standard methods to formulate and protocols to characterize protein cage-stabilized emulsions. The characterization methods are dynamic light scattering (DLS), intrinsic fluorescence spectroscopy (TF), circular dichroism (CD), and small angle X-ray scattering (SAXS). Combining these methods allows understanding of the protein cage nanostructure at the oil/water interface.

Keywords: Circular dichroism; DLS; Emulsion; Formulation; Intrinsic fluorescence; SAXS.

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References

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