Standard: Human intestinal organoids

Yalong Wang^#^{1

2

3

4}, Hanqing Lin^#⁵, Lianzheng Zhao^#¹, Fan Hong^#², Jie Hao^{6

7

8

9}, Zhen Zhang^{10

11

12}, Weiqi Sheng^{11

13}, Linhong Song¹⁴, Chu-Xia Deng¹⁵, Bing Zhao¹⁶, Jiani Cao^{6

7

8

9}, Lei Wang^{6

7

8

9}, Liu Wang^{6

7

8

9}, Lingmin Liang^{6

7

8

9}, Wenli Kelly Chen¹⁷, Chunping Yu¹⁸, Zhijian Sun¹⁹, Yingying Yang²⁰, Changlin Wang^{9

21}, Yong Zhang^{22

23}, Qiyuan Li^{9

24}, Ka Li^{9

22}, Aijin Ma^{25

26}, Tongbiao Zhao^{27

28

29

30}, Guoqiang Hua^{31

32}, Ye-Guang Chen^{33

34

35}

Affiliations

¹ The State Key Laboratory of Membrane Biology, Tsinghua-Peking Center for Life Sciences, School of Life Sciences, Tsinghua University, Beijing, 100084, China.
² Guangzhou Laboratory, Guangzhou, 510005, China.
³ Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, 510530, China.
⁴ Guangzhou Hua Yi Regeneration Technology Co., Ltd, Huangpu District, Guangzhou, 510700, China.
⁵ D1Med Technology (Shanghai) Inc, Shanghai, 201802, China.
⁶ State Key Laboratory of Stem Cell and Reproductive Biology, Institute for Stem Cell and Regeneration, Institute of Zoology, National Stem Cell Resource Center, Chinese Academy of Sciences, Beijing, 100101, China.
⁷ Beijing Institute for Stem Cell and Regenerative Medicine, Beijing, 100101, China.
⁸ University of Chinese Academy of Sciences, Beijing, 100049, China.
⁹ Institute of Clinical Science, Zhongshan Hospital, Fudan University, Shanghai, 200433, China.
¹⁰ Department of Radiation Oncology and Cancer Institute, Fudan University Shanghai Cancer Center Fudan University, Shanghai, 200032, China.
¹¹ Department of Oncology, Shanghai Medical College, Fudan University, Shanghai, 200032, China.
¹² Shanghai Key Laboratory of Radiation Oncology, Shanghai, 200032, China.
¹³ Department of Pathology, Fudan University Shanghai Cancer Center, Shanghai, 200032, China.
¹⁴ Sichuan Academy of Medical Sciences & Sichuan Provincial People's Hospital, Chengdu, 610072, China.
¹⁵ Cancer Centre, Faculty of Health Sciences, University of Macau, Macau, 999078, SAR, China.
¹⁶ State Key Laboratory of Genetic Engineering, School of Life Sciences, Fudan University, Shanghai, 200438, China.
¹⁷ China Innovation Center of Roche, Li Shi Zhen Road, Pudong, Shanghai, 201203, China.
¹⁸ Eli Lilly and Company, Pudong, Shanghai, 201203, China.
¹⁹ K2 Oncology Co., Ltd, KeChuang Street, Beijing, 100176, China.
²⁰ Qilu Pharmaceutical Co., Ltd, Jinan, 250104, China.
²¹ China National Institute of Standardization, Beijing, 100191, China.
²² Chinese Society for Stem Cell Research, Shanghai, 200032, China.
²³ HHLIFE Co., Inc, Shenzhen, 518040, China.
²⁴ China National GeneBank, Shenzhen, 518000, China.
²⁵ Institute of Clinical Science, Zhongshan Hospital, Fudan University, Shanghai, 200433, China. 783869855@qq.com.
²⁶ Beijing Technology and Business University, Beijing, 100048, China. 783869855@qq.com.
²⁷ State Key Laboratory of Stem Cell and Reproductive Biology, Institute for Stem Cell and Regeneration, Institute of Zoology, National Stem Cell Resource Center, Chinese Academy of Sciences, Beijing, 100101, China. tbzhao@ioz.ac.cn.
²⁸ Beijing Institute for Stem Cell and Regenerative Medicine, Beijing, 100101, China. tbzhao@ioz.ac.cn.
²⁹ University of Chinese Academy of Sciences, Beijing, 100049, China. tbzhao@ioz.ac.cn.
³⁰ Institute of Clinical Science, Zhongshan Hospital, Fudan University, Shanghai, 200433, China. tbzhao@ioz.ac.cn.
³¹ D1Med Technology (Shanghai) Inc, Shanghai, 201802, China. guoqianghua@fudan.edu.cn.
³² Department of Radiation Oncology and Cancer Institute, Fudan University Shanghai Cancer Center Fudan University, Shanghai, 200032, China. guoqianghua@fudan.edu.cn.
³³ The State Key Laboratory of Membrane Biology, Tsinghua-Peking Center for Life Sciences, School of Life Sciences, Tsinghua University, Beijing, 100084, China. ygchen@tsinghua.edu.cn.
³⁴ Guangzhou Laboratory, Guangzhou, 510005, China. ygchen@tsinghua.edu.cn.
³⁵ School of Basic Medicine, Jiangxi Medical College, Nanchang University, Nanchang, 330031, China. ygchen@tsinghua.edu.cn.

^# Contributed equally.

PMID: 37314549
PMCID: PMC10267020
DOI: 10.1186/s13619-023-00168-5

Editorial

Standard: Human intestinal organoids

Yalong Wang et al. Cell Regen. 2023.

. 2023 Jun 14;12(1):23.

doi: 10.1186/s13619-023-00168-5.

Authors

Affiliations

¹ The State Key Laboratory of Membrane Biology, Tsinghua-Peking Center for Life Sciences, School of Life Sciences, Tsinghua University, Beijing, 100084, China.
² Guangzhou Laboratory, Guangzhou, 510005, China.
³ Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, 510530, China.
⁴ Guangzhou Hua Yi Regeneration Technology Co., Ltd, Huangpu District, Guangzhou, 510700, China.
⁵ D1Med Technology (Shanghai) Inc, Shanghai, 201802, China.
⁶ State Key Laboratory of Stem Cell and Reproductive Biology, Institute for Stem Cell and Regeneration, Institute of Zoology, National Stem Cell Resource Center, Chinese Academy of Sciences, Beijing, 100101, China.
⁷ Beijing Institute for Stem Cell and Regenerative Medicine, Beijing, 100101, China.
⁸ University of Chinese Academy of Sciences, Beijing, 100049, China.
⁹ Institute of Clinical Science, Zhongshan Hospital, Fudan University, Shanghai, 200433, China.
¹⁰ Department of Radiation Oncology and Cancer Institute, Fudan University Shanghai Cancer Center Fudan University, Shanghai, 200032, China.
¹¹ Department of Oncology, Shanghai Medical College, Fudan University, Shanghai, 200032, China.
¹² Shanghai Key Laboratory of Radiation Oncology, Shanghai, 200032, China.
¹³ Department of Pathology, Fudan University Shanghai Cancer Center, Shanghai, 200032, China.
¹⁴ Sichuan Academy of Medical Sciences & Sichuan Provincial People's Hospital, Chengdu, 610072, China.
¹⁵ Cancer Centre, Faculty of Health Sciences, University of Macau, Macau, 999078, SAR, China.
¹⁶ State Key Laboratory of Genetic Engineering, School of Life Sciences, Fudan University, Shanghai, 200438, China.
¹⁷ China Innovation Center of Roche, Li Shi Zhen Road, Pudong, Shanghai, 201203, China.
¹⁸ Eli Lilly and Company, Pudong, Shanghai, 201203, China.
¹⁹ K2 Oncology Co., Ltd, KeChuang Street, Beijing, 100176, China.
²⁰ Qilu Pharmaceutical Co., Ltd, Jinan, 250104, China.
²¹ China National Institute of Standardization, Beijing, 100191, China.
²² Chinese Society for Stem Cell Research, Shanghai, 200032, China.
²³ HHLIFE Co., Inc, Shenzhen, 518040, China.
²⁴ China National GeneBank, Shenzhen, 518000, China.
²⁵ Institute of Clinical Science, Zhongshan Hospital, Fudan University, Shanghai, 200433, China. 783869855@qq.com.
²⁶ Beijing Technology and Business University, Beijing, 100048, China. 783869855@qq.com.
²⁷ State Key Laboratory of Stem Cell and Reproductive Biology, Institute for Stem Cell and Regeneration, Institute of Zoology, National Stem Cell Resource Center, Chinese Academy of Sciences, Beijing, 100101, China. tbzhao@ioz.ac.cn.
²⁸ Beijing Institute for Stem Cell and Regenerative Medicine, Beijing, 100101, China. tbzhao@ioz.ac.cn.
²⁹ University of Chinese Academy of Sciences, Beijing, 100049, China. tbzhao@ioz.ac.cn.
³⁰ Institute of Clinical Science, Zhongshan Hospital, Fudan University, Shanghai, 200433, China. tbzhao@ioz.ac.cn.
³¹ D1Med Technology (Shanghai) Inc, Shanghai, 201802, China. guoqianghua@fudan.edu.cn.
³² Department of Radiation Oncology and Cancer Institute, Fudan University Shanghai Cancer Center Fudan University, Shanghai, 200032, China. guoqianghua@fudan.edu.cn.
³³ The State Key Laboratory of Membrane Biology, Tsinghua-Peking Center for Life Sciences, School of Life Sciences, Tsinghua University, Beijing, 100084, China. ygchen@tsinghua.edu.cn.
³⁴ Guangzhou Laboratory, Guangzhou, 510005, China. ygchen@tsinghua.edu.cn.
³⁵ School of Basic Medicine, Jiangxi Medical College, Nanchang University, Nanchang, 330031, China. ygchen@tsinghua.edu.cn.

^# Contributed equally.

PMID: 37314549
PMCID: PMC10267020
DOI: 10.1186/s13619-023-00168-5

Abstract

Organoids have attracted great interest for disease modelling, drug discovery and development, and tissue growth and homeostasis investigations. However, lack of standards for quality control has become a prominent obstacle to limit their translation into clinic and other applications. "Human intestinal organoids" is the first guideline on human intestinal organoids in China, jointly drafted and agreed by the experts from the Chinese Society for Cell Biology and its branch society: the Chinese Society for Stem Cell Research. This standard specifies terms and definitions, technical requirements, test methods, inspection rules for human intestinal organoids, which is applicable to quality control during the process of manufacturing and testing of human intestinal organoids. It was originally released by the Chinese Society for Cell Biology on 24 September 2022. We hope that the publication of this standard will guide institutional establishment, acceptance and execution of proper practical protocols and accelerate the international standardization of human intestinal organoids for applications.

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Conflict of interest statement

The authors declare no competing financial interests. Y.-G.C. is the Editor‑in‑Chief of Cell Regeneration. He was not involved in the review or decision related to this manuscript. This work was not sponsored by any commercial organizations, and all the other authors declare that they have no competing interests.

References

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1. Barker N, van Es JH, Kuipers J, Kujala P, van den Born M, Cozijnsen M, et al. Identification of stem cells in small intestine and colon by marker gene Lgr5. Nature. 2007;449(7165):1003–1007. doi: 10.1038/nature06196. - DOI - PubMed
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Standard: Human intestinal organoids

Affiliations

Standard: Human intestinal organoids

Authors

Affiliations

Abstract

Conflict of interest statement

References

Publication types

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