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. 2023 May 5;11(5):1214.
doi: 10.3390/microorganisms11051214.

Experimental Studies of the Liposomal Form of Lytic Mycobacteriophage D29 for the Treatment of Tuberculosis Infection

Vadim Vadimovich Avdeev  1 , Victor Vladimirovich Kuzin  1 , Mikhail Aleksandrovich Vladimirsky  1 , Irina Anatol'evna Vasilieva  1
Affiliations

Experimental Studies of the Liposomal Form of Lytic Mycobacteriophage D29 for the Treatment of Tuberculosis Infection

Vadim Vadimovich Avdeev et al. Microorganisms. .

Abstract

We have studied the antimycobacterial efficacy of the liposomal preparation of mycobacteriophage D29 on models of tuberculous granuloma in vitro and in the experiment on laboratory mice of the relatively resistant strain C57BL/6, infected with the virulent strain of M. tuberculosis H37Rv. We have shown the preparation of liposomal preparation of the lytic mycobacteriophages and its characteristics. The experiments showed a significant lytic effect of the liposomal form of mycobacteriophage D29 both on the model of tuberculous granuloma formed by human blood mononuclear cells in vitro, which is formed in the presence of Mycobacterium tuberculosis and on the model of tuberculous infection in C57BL/6 mice. Keywords: mycobacteriophage D29, M. tuberculosis, liposomes, tuberculous granuloma in vitro, tuberculosis infection and its treatment.

Keywords: TB granuloma; experiments in vivo; liposomes; lytic mycobacteriophages; tuberculosis treatment.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Chromatography peak image of lytic mycobacteriophage D29. formula image Optical density [u] formula image Conductivity [mS/cm].
Figure 2
Figure 2
Image of TB granuloma on the day 14.
Figure 3
Figure 3
Electron microscopy of a liposomal preparation of mycobacteriophage D29 obtained by extrusion (liposome diameter 0.8 microns). Magnification 1:25,000. Scale—500 nm.
Figure 4
Figure 4
Electron microscopy of a liposomal preparation of mycobacteriophage D29 obtained by column chromatography. Scale—200 nm. The average size of liposomes is 0.25 microns.
Figure 5
Figure 5
Comparison of culture results obtained in an in vitro TB granuloma experiment formed by human blood mononuclear cells. (A) positive control, (B) liposomal preparation of mycobacteriophage D29, and (C) non-encapsulated (free) preparation of mycobacteriophage D29.
Figure 6
Figure 6
Histological picture of changes in the lung tissue of the mouse. (A) diffuse inflammation of the lung tissue, (B) perivascular fibrosis, and plethora.
Figure 7
Figure 7
Intact lung tissue.
See this image and copyright information in PMC

References

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