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. 2023 Sep:71:103141.
doi: 10.1016/j.scr.2023.103141. Epub 2023 Jun 10.

Generation of a transgenic mouse embryonic stem cell line overexpressing DNMT1

Sumana Choudhury  1 , Anuhya Anne  1 , Purnima P Pradhan  2 , K Naga Mohan  3
Affiliations
Free article

Generation of a transgenic mouse embryonic stem cell line overexpressing DNMT1

Sumana Choudhury et al. Stem Cell Res. 2023 Sep.
Free article

Abstract

DNMT1 overexpression is reported in disorders like schizophrenia, bipolar, epilepsy and multiple cancer types. Here, we used non-homologous recombination to generate R1Dnmt1WT-1, a mouse embryonic stem cell (ESC) line carrying a Dnmt1 cDNA transgene to achieve about two-fold overexpression. This ESC line showed increased transcript levels of Sox2 pluripotency marker. R1Dnmt1WT-1 embryoid bodies showed increased levels of Lefty1 (endoderm), Tbxt and Acta2 (mesoderm), and Pax6 (ectoderm) transcripts. This new line showed normal karyotype and microsatellite profiles making it useful in studying carcinogenesis and abnormal neurogenesis due to DNMT1 overexpression.

Keywords: Cancers; DNMT1 overexpression; Schizophrenia.

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Conflict of interest statement

Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

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