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Review
. 2023 May 30:11:1217067.
doi: 10.3389/fbioe.2023.1217067. eCollection 2023.

Physical processing for decellularized nerve xenograft in peripheral nerve regeneration

Ming-Wei Hsu  1 , Szu-Han Chen  1   2   3 , Wan-Ling Tseng  4 , Kuo-Shu Hung  1 , Tzu-Chun Chung  5 , Sheng-Che Lin  6 , Jahyun Koo  7 , Yuan-Yu Hsueh  1   2   3
Affiliations
Review

Physical processing for decellularized nerve xenograft in peripheral nerve regeneration

Ming-Wei Hsu et al. Front Bioeng Biotechnol. .

Abstract

In severe or complex cases of peripheral nerve injuries, autologous nerve grafts are the gold standard yielding promising results, but limited availability and donor site morbidity are some of its disadvantages. Although biological or synthetic substitutes are commonly used, clinical outcomes are inconsistent. Biomimetic alternatives derived from allogenic or xenogenic sources offer an attractive off-the-shelf supply, and the key to successful peripheral nerve regeneration focuses on an effective decellularization process. In addition to chemical and enzymatic decellularization protocols, physical processes might offer identical efficiency. In this comprehensive minireview, we summarize recent advances in the physical methods for decellularized nerve xenograft, focusing on the effects of cellular debris clearance and stability of the native architecture of a xenograft. Furthermore, we compare and summarize the advantages and disadvantages, indicating the future challenges and opportunities in developing multidisciplinary processes for decellularized nerve xenograft.

Keywords: decellularized nerve xenograft; freeze-thaw; immersion and agitation; perfusion; peripheral nerve regeneration; physical processing; sonication; supercritical fluids.

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Conflict of interest statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Figures

FIGURE 1
FIGURE 1
The physical processing methods for tissue decellularization of nerve xenograft, including freezing and thawing cycle, sonication, immersion and agitation, perfusion and supercritical fluids.
See this image and copyright information in PMC

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