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. 2023 Jun 16;18(6):e0287331.
doi: 10.1371/journal.pone.0287331. eCollection 2023.

The mechanism of action of Ophiocordyceps sinensis mycelia for prevention of acute lung injury based on non-targeted serum metabolomics

Jinna Zhou  1   2 , Na Pi  3 , Yingqi Guo  4 , Xinyi He  1 , Jinhu Wang  2 , Run Luo  1 , Mu Wang  5 , Hong Yu  1
Affiliations

The mechanism of action of Ophiocordyceps sinensis mycelia for prevention of acute lung injury based on non-targeted serum metabolomics

Jinna Zhou et al. PLoS One. .

Abstract

Ophiocordyceps sinensis is a fungus with medicinal value in treating lung diseases, but no study has reported how to prevent acute lung injury using this fungus. The mice were divided into normal, model, positive control, and O. sinensis groups to observe lung histopathological sections and transmission electron microscopy, along with liquid chromatography-mass spectrometry and hematoxylin and eosin (H&E) staining to closely identify structural differences resulting from destruction between the groups. The results of the H&E staining showed that, compared with the normal group, the model group showed alveolar collapse. Compared with the model group, the infiltration of inflammatory cells in the alveolar cavity of the O. sinensis group was significantly reduced. Mitochondrial plate-like cristae were observed in type II alveolar cells of the normal group, with normal coloration of the mitochondrial matrix. Type II alveolar cells in the model group showed obvious edema. The statuses of type II alveolar cells in the O. sinensis and positive groups were similar to that in the normal group. Twenty-nine biomarkers and 10 related metabolic pathways were identified by serum metabolomics screening. The results showed that O. sinensis mycelia had a significant effect on the prevention of lipopolysaccharide-induced inflammation.

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Conflict of interest statement

The authors have declared that no competing interests exist.

Figures

Fig 1
Fig 1. Presents the lung coefficient (n = 5).
LPS group vs. NS group, **** P <0.001; LPS group vs. OS group, * P <0.01, LPS group vs. DXM group, * P <0.01; NS group vs. OS group, ### P <0.05, NS group vs. DXM group, ### P <0.05.
Fig 2
Fig 2. Representative H&E-stained lung sections (n = 5), scar bar = 20 μm.
Fig 3
Fig 3. Ultrastructural changes in the lung tissue of mice in each group.
LB, platelet vesicles; M, mitochondria; and↑ endoplasmic reticulum.
Fig 4
Fig 4
(a) Typical peak chromatograms of each group of samples. (b) Metabolite classification bar chart.
Fig 5
Fig 5
(a) Score chart of the PCA analysis model; (b) score chart of the PLS-DA analysis model of LPS_NS; (c) score chart of the PLS-DA analysis model of LPS_OS; and (d) score chart of the PLS-DA analysis model of NS_OS.
Fig 6
Fig 6
(a) Volcano map of differential metabolites of LPS_NS; (b) volcano map of differential metabolites of LPS_OS; and (c) bubble diagram for metabolic pathway enrichment analysis.
See this image and copyright information in PMC

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