Frontiers and techniques in plant gene regulation

Abstract

Understanding plant gene regulation has been a priority for generations of plant scientists. However, due to its complex nature, the regulatory code governing plant gene expression has yet to be deciphered comprehensively. Recently developed methods-often relying on next-generation sequencing technology and state-of-the-art computational approaches-have started to further our understanding of the gene regulatory logic used by plants. In this review, we discuss these methods and the insights into the regulatory code of plants that they can yield.

Keywords: Cis-regulatory elements; DNA accessibility; Massively parallel reporter assay; Plant STARR-seq; Plant gene regulation; Single-cell sequencing; Single-molecule chromatin assay.