HPTLC and GC-MS finger-printing of two potential multifunctional siddha tailams: Mathan and maha megarajanga tailam

Abstract

The Siddha system of medicine is an ancient medical lineage that is practiced primarily in the southern part of India. Siddha system of medicine has been in practice for thousands of years with documented evidence dating back to the 6th century BCE. According to siddha system of medicine's basic fundamental principle, the human body is made up of 96 thathuvam (primary components), which encompass physical, physiological, psychological, and intellectual aspects. Medicine (marunthu) is classified as a wide range of internal and external medicines. The major components of its medical formulations include plant parts, minerals and animal products. Various methods were carried out for the purification process to eliminate the toxins. Choornam, Guligai, Tailam, Parpam, Chendooram, Kattu, Pasai and Poochu are the most common medicines used in Siddha system of medicine for the treatment of various diseases. The pathophysiological classification of diseases is elaborated in detail in the classical Siddha literature. Siddha system of medicine plays an important role in protecting people from diseases such as COVID-19 by providing immune-protecting and immune-boosting medicines in today's world. Mathan tailam and maha megarajanga tailam are the two unique preparations used widely for various skin diseases including chronic wounds and burns. Scientific validation of both medicines will help in understanding their effectiveness against a typical wound condition. In the present study physio-chemical and phytochemical, HPTLC, and GC-MS analyses were carried out and discussed in detail on the multifunctional properties exhibited in the patient communities.

Keywords: Mathan tailam; Megarajanga tailam; Oil formulation; Siddha medicine; Wound healing.

Fig. 1

MT Thin Layer Chromatography observations. (A)Under short UV λ = 254 nm, (B) Under long UV λ = 366 nm, (C) Under white light after derivatisation λ = 520 nm.

Fig. 2

(A-B) MT HPTLC Chromatogram and fingerprint profile @ 254 nm, (C-D) HPTLC Chromatogram and fingerprint profile @ 366 nm, (E-F) HPTLC Chromatogram and Peak table @ 520 nm.

Fig. 3

MMRT (A) Under short UV λ = 254 nm, (B) Under long UV λ = 366 nm, (C) Under white light after derivatisation λ = 520 nm.

Fig. 4

MMRT (A-B) HPTLC Chromatogram and fingerprint profile @ 254 nm, (C-D) HPTLC Chromatogram and fingerprint profile @ 366 nm, (E-F) HPTLC Chromatogram and Peak table @ 520 nm.