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. 2023 Sep;239(5):1935-1953.
doi: 10.1111/nph.19073. Epub 2023 Jun 19.

Ptr1 and ZAR1 immune receptors confer overlapping and distinct bacterial pathogen effector specificities

Ye Jin Ahn #  1 , Haseong Kim #  1   2 , Sera Choi #  1 , Carolina Mazo-Molina  3   4 , Maxim Prokchorchik  1 , Ning Zhang  3   4 , Boyoung Kim  2   5 , Hyunggon Mang  1 , Naio Koehler  6 , Jieun Kim  1 , Soeui Lee  2   5 , Hayeon Yoon  1 , Doil Choi  2   5 , Min-Sung Kim  1 , Cécile Segonzac  2   5   7   8 , Gregory B Martin  3   4 , Alex Schultink  6 , Kee Hoon Sohn  1   2   8   9
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Free article

Ptr1 and ZAR1 immune receptors confer overlapping and distinct bacterial pathogen effector specificities

Ye Jin Ahn et al. New Phytol. 2023 Sep.
Free article

Erratum in

Abstract

Some nucleotide-binding and leucine-rich repeat receptors (NLRs) indirectly detect pathogen effectors by monitoring their host targets. In Arabidopsis thaliana, RIN4 is targeted by multiple sequence-unrelated effectors and activates immune responses mediated by RPM1 and RPS2. These effectors trigger cell death in Nicotiana benthamiana, but the corresponding NLRs have yet not been identified. To identify N. benthamiana NLRs (NbNLRs) that recognize Arabidopsis RIN4-targeting effectors, we conducted a rapid reverse genetic screen using an NbNLR VIGS library. We identified that the N. benthamiana homolog of Ptr1 (Pseudomonas tomato race 1) recognizes the Pseudomonas effectors AvrRpt2, AvrRpm1, and AvrB. We demonstrated that recognition of the Xanthomonas effector AvrBsT and the Pseudomonas effector HopZ5 is conferred independently by the N. benthamiana homolog of Ptr1 and ZAR1. Interestingly, the recognition of HopZ5 and AvrBsT is contributed unequally by Ptr1 and ZAR1 in N. benthamiana and Capsicum annuum. In addition, we showed that the RLCK XII family protein JIM2 is required for the NbZAR1-dependent recognition of AvrBsT and HopZ5. The recognition of sequence-unrelated effectors by NbPtr1 and NbZAR1 provides an additional example of convergently evolved effector recognition. Identification of key components involved in Ptr1 and ZAR1-mediated immunity could reveal unique mechanisms of expanded effector recognition.

Keywords: Nicotiana benthamiana; NLR; convergent evolution; effector recognition; plant immune receptors.

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References

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