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. 2023 Jun 20;23(1):209.
doi: 10.1186/s12876-023-02842-z.

The effect of Trolox on the rabbit anal sphincterotomy repair

Arash Sarveazad  1   2 , Abazar Yari  3 , Farnad Imani  4 , Farimah Fayyaz  1 , Marjan Mokhtare  1 , Arash Babaei-Ghazani  5   6 , Mahmoud Yousefifard  7 , Shahriar Sarveazad  1 , Siavash Assar  8 , Jebreil Shamseddin  9 , Mansour Bahardoust  10
Affiliations

The effect of Trolox on the rabbit anal sphincterotomy repair

Arash Sarveazad et al. BMC Gastroenterol. .

Abstract

Introduction: Fecal incontinence (FI) is caused by external anal sphincter injury. Vitamin E is a potential strategy for anal sphincter muscle repair via its antioxidant, anti-inflammatory, anti-fibrotic, and protective properties against myocyte loss. Thus, we aimed to evaluate the water-soluble form of vitamin E efficacy in repairing anal sphincter muscle defects in rabbits.

Methods: Twenty-one male rabbits were equally assigned to the intact (without any intervention), control (sphincterotomy), and Trolox (sphincterotomy + Trolox administration) groups. Ninety days after sphincterotomy, the resting and squeeze pressures were evaluated by manometry, and the number of motor units in the sphincterotomy site was calculated by electromyography. Also, the amount of muscle and collagen in the injury site was investigated by Mallory's trichrome staining.

Results: Ninety days after the intervention, the resting and squeeze pressures in the intact and Trolox groups were significantly higher than in the control group (P = 0.001). Moreover, the total collagen percentage of the sphincterotomy site was significantly lower in the Trolox group than in the control group (P = 0.002), and the total muscle percentage was significantly higher in the Trolox group compared to the control group (P = 0.001). Also, the motor unit number was higher in the Trolox group than in the control group (P = 0.001).

Conclusion: Trolox administration in the rabbit sphincterotomy model can decrease the amount of collagen and increase muscle, leading to improved anal sphincter electromyography and manometry results. Therefore, Trolox is a potential treatment strategy for FI.

Keywords: External anal sphincter; Fecal incontinence; Rabbit; Trolox.

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Conflict of interest statement

The authors declare that they have no competing interests.

Figures

Fig. 1
Fig. 1
(A) Resting and (B) Maximum Strength After stimulation (squeeze) pressure in the study groups. Data are presented as mean ± SEM (n = 7 per group). †† Significant level at p-value < 0.0001 with the control group. ## Significant level at p-value < 0.0001 with the Trolox group
Fig. 2
Fig. 2
Collagen and muscle amount in the study groups. (A) Intact, (B) Control, and (C) Trolox group. (D) Percentage of collagen and (E) muscle. Data are presented as mean ± SEM (n = 7 per group). †† Significant level at p-value < 0.0001 with the control group. ## Significant level at p-value < 0.0001 with the Trolox group
Fig. 3
Fig. 3
Motor unite number in the study groups. (A) Intact, (B) Control, and (C) Trolox group. (D) Data are presented as means ± SEM (n = 7 animals per group). **Significant level at p < 0.0001 with the Sphincterotomy group (intact animals). ## Significant level at p < 0.0001 with the Control group
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