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Review
. 2023 Jun 20;61(6):e0089322.
doi: 10.1128/jcm.00893-22. Epub 2023 Jun 20.

The Brief Case: a White-Colony-Producing Clostridioides difficile Ribotype 020 Strain

Korakrit Imwattana  1   2 , Niraj Shivaperumal  1 , Teera Leepattarakit  2 , Pattarachai Kiratisin  2 , Daniel R Knight  1   3 , Thomas V Riley  1   3   4   5
Affiliations
Review

The Brief Case: a White-Colony-Producing Clostridioides difficile Ribotype 020 Strain

Korakrit Imwattana et al. J Clin Microbiol. .
No abstract available

Keywords: ChromID agar; Clostridioides difficile; diagnosis; esculin hydrolysis.

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Conflict of interest statement

The authors declare a conflict of interest. T.V.R. has received grants from Cepheid, Merck, Otsuka, Roche, Sanofi and Summit for work outside that in this report. All other authors declare no conflicts of interest.

Figures

FIG 1
FIG 1
Phenotypic characteristics of C. difficile strain MAR214. (A and B) MAR214 produced white colonies on ChromID agar (A) and typical, but relatively small, C. difficile colonies on blood agar, which fluoresced under UV light (B). (C) MAR214 (marked with a red asterisk) produced a dense GDH band and a faint toxin band with C. Diff Quik Chek Complete (compared to a positive control accompanying the test kit). (D) MAR214 (marked with a red asterisk) was positive for proline-aminopeptidase. (E) Growth kinetics of C. difficile MAR214 were compared to those of four reference strains. Optical density at 600 nm (OD600) for each C. difficile strain was recorded up to 36 h. C. difficile MAR214 (red) had the lowest OD600 at the stationary phase (average OD600 of 0.38 compared to 0.58, P < 0.0001). Other tubes (from left to right): a clinical isolate of normal C. difficile; a clinical isolate of Bacteroides fragilis; 0.85% NaCl solution; C. difficile ATCC 700057.
FIG 2
FIG 2
Esculin transport and hydrolysis in C. difficile. (A) Phosphotransferase system (PTS) enzyme I (PTS EI) phosphorylates PTS enzyme II (PTS EII), which is specific to each carbohydrate source. (B) β-Glucoside-specific PTS EII phosphorylates esculin and transports esculin-phosphate into the cell. (C) Esculinase (6-phospho-β-d-glucosidase) hydrolyses esculin-phosphate into glucose-phosphate and esculetin. Glucose-phosphate further undergoes glycolysis.
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References

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