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. 2023 Jul 18;12(7):e0026423.
doi: 10.1128/mra.00264-23. Epub 2023 Jun 20.

Complete Genome of Agrobacterium fabrum Strain 1D1416

Affiliations

Complete Genome of Agrobacterium fabrum Strain 1D1416

Diaa Alabed et al. Microbiol Resour Announc. .

Abstract

This work reports the draft genome of Agrobacterium fabrum strain 1D1416. The assembled genome is composed of a 2,837,379-bp circular chromosome, a 2,043,296-bp linear chromosome, a 519,735-bp AT1 plasmid, a 188,396-bp AT2 plasmid, and a 196,706-bp Ti virulence plasmid. The nondisarmed strain produces gall-like structures in citrus tissue.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

FIG 1
FIG 1
Gall formation and stable DsRed expression in citrus epicotyl tissue transformed with wild-type Agrobacterium fabrum strain 1D1416 harboring the binary vector pCTAGV-KCN3. Left panel, gall viewed under white light. Right panel, same gall displaying DsRed expression. The binary plasmid and the protocol used for citrus transformation were previously published (1).

References

    1. de Oliveira MLP, Stover E, Thomson JG. 2015. The codA gene as a negative selection marker in Citrus. Springerplus 4:264. doi: 10.1186/s40064-015-1047-y. - DOI - PMC - PubMed
    1. Wise AA, Liu Z, Binns AN. 2006. Nucleic acid extraction from Agrobacterium strains, p 67–76. In Wang K (ed), Agrobacterium protocols, 2nd ed, vol 1. Humana Press, Totowa, NJ. doi: 10.1385/1-59745-130-4:67. - DOI - PubMed
    1. Qiagen. 2013–2023. CLC Genomics Workbench 8.5. Qiagen, Redwood City, CA. https://digitalinsights.qiagen.com/products-overview/discovery-insights-....
    1. Chin C-S, Alexander DH, Marks P, Klammer AA, Drake J, Heiner C, Clum A, Copeland A, Huddleston J, Eichler EE, Turner SW, Korlach J. 2013. Nonhybrid, finished microbial genome assemblies from long-read SMRT sequencing data. Nat Methods 10:563–569. doi: 10.1038/nmeth.2474. - DOI - PubMed
    1. Brettin T, Davis JJ, Disz T, Edwards RA, Gerdes S, Olsen GJ, Olson R, Overbeek R, Parrello B, Pusch GD, Shukla M, Thomason JA, III, Stevens R, Vonstein V, Wattam AR, Xia F. 2015. RASTtk: a modular and extensible implementation of the RAST algorithm for building custom annotation pipelines and annotating batches of genomes. Sci Rep 5:8365. doi: 10.1038/srep08365. - DOI - PMC - PubMed