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. 2023 Jul;29(7):1465-1469.
doi: 10.3201/eid2907.221229.

Detecting, Quantifying, and Isolating Monkeypox Virus in Suspected Cases, Spain

Marta E Álvarez Argüelles, Zulema Pérez Martínez, Susana Rojo Alba, Jose María González Alba, Ana María Fernandez-Verdugo, Isabel Costales González, Garbriel Martín Rodríguez, Jose Antonio Boga Riveiro, Mario Margolles Martins, Santiago Melón García

Detecting, Quantifying, and Isolating Monkeypox Virus in Suspected Cases, Spain

Marta E Álvarez Argüelles et al. Emerg Infect Dis. 2023 Jul.

Abstract

When a monkeypox virus outbreak began in several parts of the world in May 2022, timely and accurate diagnosis became mandatory. In our laboratory, a real-time quantitative PCR was designed and evaluated in several patient samples and compared with isolation results. Genomic viral load was related to virus viability.

Keywords: Spain; cell culture; monkeypox virus; mpox; orthopoxvirus; real-time PCR; sexually transmitted infections; viruses.

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Figures

Figure 1
Figure 1
Cytopathic effect in monkeypox virus–infected cells from patients in Asturias, Spain. A) MRC-5; B) Vero E6; C) A549; D) LLC-MK2. Original magnification ×10.
Figure 2
Figure 2
Phylogenetic relationships of monkeypox virus strains from patients in Asturias, Spain (bold), and reference strains from GenBank and GISAID (https://www.gisaid.org). Alignment has 57 sequences with 262 nucleotides. Numbers in nodes are SH-aLRT support (%)/ultrafast bootstrap support (%). Scale bar indicates number of base substitutions per site.
See this image and copyright information in PMC

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