Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2023 Sep;133(3):254-264.
doi: 10.1111/bcpt.13919. Epub 2023 Jul 9.

The antioxidant/anti-inflammatory protective effects of rutin against ethanol-induced testicular perturbation in rats: Involvement of the immunosuppressive indoleamine 2,3-dioxygenase

Azubuike Peter Ebokaiwe  1 , Doris Olachi Obasi  1 , Sharon Oluchi Osawe  2 , Rex Clovis C Njoku  1
Affiliations
Free article

The antioxidant/anti-inflammatory protective effects of rutin against ethanol-induced testicular perturbation in rats: Involvement of the immunosuppressive indoleamine 2,3-dioxygenase

Azubuike Peter Ebokaiwe et al. Basic Clin Pharmacol Toxicol. 2023 Sep.
Free article

Abstract

This study investigated the influence of rutin against EtOH-induced testicular impairment in rats and the involvement of the indole-aminergic pathway. Four groups of eight rats each were orally exposed to drinking water (Group 1), EtOH (5 g/kg bwt, Group 2), R (5 g/kg bwt, Group 3), and EtOH + R (5 g/kg bwt + 50 mg/kg bwt, Group 4) via gavage for 15 days. Results showed that exposure to EtOH significantly (p < 0.0001) reduced the testicular antioxidant system and increased lipid peroxidation (LPO) relative to control. We observed a significant (p < 0.0001) increase in the inflammatory biomarkers, with attendant disruption in the testicular histological structure and concomitant elevation in the activities of indoleamine 2,3-dioxygenase (IDO), in comparison with control and no noticeable effects in tryptophan 2,3-dioxygenase (TDO) activity across the groups. Rutin-only exposed group did not show any alteration in the measured parameters when compared with the control. Rutin co-exposure augmented the antioxidant system, prevented histological damage, reduced LPO and inflammation, and thus, lowered EtOH-mediated increase in IDO activity, compared with control. Overall, these findings reveal the involvement of the indole-aminergic pathway in rutin's protective influence against EtOH-induced testicular impairment in rats.

Keywords: alcohol; indoleamine 2,3-dioxygenase; male rats; oxidative-inflammatory stress; testes.

PubMed Disclaimer

References

REFERENCES

    1. Reddy KP, Reddy PS. Testicular and epididymal toxicity induced by benzo(a)pyrene, alcohol, and their combination in Wistar rats. Toxicol Res. 2016;5(2):420-433. doi:10.1039/C5TX00420A
    1. Akomolafe SF, Oboh G, Akindahunsi AA, Afolayan AJ. Ethanol­induced male infertility: Effects of aqueous leaf extract of Tetracarpidium conophorum. Andrologia. 2017;49(10):e12759. doi:10.1111/and.12759
    1. Radhakrishnakartha H, Appu AP, Madambath I. Reversal of alcohol induced testicular hyperlipidemia by supplementation of ascorbic acid and its comparison with abstention in male Guinea pigs. J Basic Clin Physiol Pharmacol. 2014;25(1):117-124. doi:10.1515/jbcpp-2012-0056
    1. World Health Organisation, WHO Global Status Report on Alcohol. 2004. http://www.who.int/entity/substance_abuse/publication/global_status_repo...
    1. Dosumu OO, Osinubi AAA, Duru FIO. Alcohol-induced testicular damage: can abstinence equal recovery? Middle East Fertil Soc J. 2014;19(3):221-228. doi:10.1016/j.mefs.2014.01.003