Indicators of thermal comfort and nitrogen digestibility as a function of digestible arginine: lysine ratios in the diet of laying Japanese quails raised in hot weather

Abstract

The objective with this study was to determine the influence of the digestible arginine: lysine ratio in the diet of laying Japanese quails raised in hot weather on the physiological variables of thermoregulation, time in tonic immobility, behavior and nitrogen utilization. A total of 240 laying quails (Coturnix coturnix japonica) with 40 weeks of age were used in a completely randomized design with five treatments and six replications containing eight quails per experimental unit. The studied digestible arginine: lysine ratios were 110%; 120%, 130%, 140% and 150%, for 63 days divided into three periods of 21 days each. There was a quadratic effect (p < 0.05) for the variables average surface temperature, average body temperature, core to surface thermal gradient, ambient to surface temperature gradient observed in the morning. In the afternoon, a quadratic effect (p < 0.05) of the arginine: lysine ratio was observed for variables average surface temperature, average body temperature, core to surface thermal gradient, ambient to surface thermal gradient, back temperature, head temperature and respiratory frequency. There was no significant effect (p > 0.05) of arginine: lysine ratios on the frequency of behaviors, except drinking and panting (p < 0.05), for which 130% ratio provided the lowest frequencies of these behaviors. There was a quadratic effect (p < 0.05) for the parameters of nitrogen retention, nitrogen excreted and nitrogen retention efficiency. Nitrogen intake and excreta moisture were not influenced by the different relationships studied (p > 0.05). The 130% arginine: lysine ratio corresponding to 0.131 g/kg/feed of arginine improves physiological responses related to thermoregulation and decreases the expression of behaviors correlated to heat stress, also meeting the best estimated ratio for nitrogen retention in the diet of quails laying Japanese raised in hot weather.

Keywords: Amino acids; Behavior; Ideal protein; Thermal comfort; Thermoregulation.