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. 2023:2691:351-369.
doi: 10.1007/978-1-0716-3331-1_25.

Engineering Cell Lines for Specific Human Leukocyte Antigen Presentation

Dongbin Jin  1 , Khai Lee Loh  2 , Tima Shamekhi  1 , Yi Tian Ting  2 , Terry C C Lim Kam Sian  1   3 , James Roest  4 , Joshua D Ooi  2 , Julian P Vivian #  5   6 , Pouya Faridi #  1   3
Affiliations

Engineering Cell Lines for Specific Human Leukocyte Antigen Presentation

Dongbin Jin et al. Methods Mol Biol. 2023.

Abstract

Epitope-specific immunotherapies have enabled the targeted treatment of a variety of diseases, ranging from cancer, infection, and autoimmune disorders. For CD8+ T cell-based therapies, the precise identification of immunogenic peptides presented by human leukocyte antigen (HLA) class I is essential which can be achieved by immunopeptidomics. Here, using lentivirus-mediated transduction and cell sorting approaches, we present a method to engineer a cell line that does not express its native HLA but instead expresses an HLA of interest (in this instance HLA-A*02:01). This technique can be used to elucidate the immunopeptidome of cell lines expressing different HLAs.

Keywords: Cancer; Cell lines; Gene editing; HLA; Immunopeptidomics; Immunotherapy; Lentivirus; Neoantigens; Transduction.

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