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. 2023 Apr 10.
doi: 10.1016/j.vacun.2023.04.002. Online ahead of print.

The polymorphisms of TNF-α related-gene and antibody production following third dose of COVID-19 vaccination: A pilot study

Phey Liana  1 , Zen Hafy  2 , Soilia Fertilita  3 , Ella Amalia  4 , Veny Larasati  3 , Tungki Pratama Umar  5
Affiliations

The polymorphisms of TNF-α related-gene and antibody production following third dose of COVID-19 vaccination: A pilot study

Phey Liana et al. Vacunas. .

Abstract
in English, Spanish

Introduction: Vaccination is one of the most pertinent prevention strategies for the Coronavirus Disease 2019 (COVID-19) pandemic. Several factors, both intrinsic (particularly genetic) and extrinsic, can influence vaccine efficacy. However, very little research has been conducted into the genetic component's impact on immunogenicity following COVID-19 vaccination. Therefore, we present the antibody formation in thirteen people who received a third vaccination (booster) dose of the Moderna mRNA-1273 vaccine and the differences in the polymorphism Tumor Necrosis Factor-Alpha (TNF-α) related genes in this population.

Methods: Our study included 13 participants with no comorbidities or a history of COVID-19 infection. The Chemiluminescent Microparticle Immunoassay (CMIA) was used to measure antibody production in serum. Polymorphism was recognized using the polymerase chain reaction (PCR) amplification technique.

Results: In this study, TNF-α related gene (GG) significantly influenced the formation of the antiSARS-CoV-2 spike protein IgG antibody level (p = 0.005) in our sample.

Conclusion: Although the polymorphism of the cytokine gene, particularly TNF-α, seems to influence antibody levels in our study population, a more comprehensive analysis is required for better generalization due to the nature of our pilot study.

Introducción: La vacunación es una de las estrategias de prevención más pertinentes ante la pandemia de Enfermedad del coronavirus 2019 (COVID-19). Varios factores, tanto intrínsecos (particularmente genéticos) como extrínsecos, pueden influir en la eficacia de la vacuna. Sin embargo, se ha realizado muy poca investigación sobre el impacto del componente genético en la inmunogenicidad después de la vacunación contra el COVID-19. Por lo tanto, presentamos la formación de anticuerpos en trece personas que recibieron una tercera dosis de vacunación (refuerzo) de la vacuna Moderna mRNA-1273 y las diferencias en los genes relacionados con el polimorfismo el factor de necrosis tumoral alfa (TNF-α) en esta población.

Métodos: Nuestro estudio incluyó a 13 participantes sin comorbilidades o antecedentes de infección por COVID-19. Se utilizó el inmunoanálisis de micropartículas quimioluminiscentes (CMIA) para medir la producción de anticuerpos en suero. El polimorfismo se reconoció utilizando la técnica de amplificación de la reacción en cadena de la polimerasa (PCR).

Resultados: En este estudio, el gen relacionado con TNF-α (GG) influyó significativamente en la formación del nivel de anticuerpos IgG de proteína de punta antiSARS-CoV-2 (p = 0,005) en nuestra muestra.

Conclusiones: Aunque el polimorfismo del gen de la citoquina, particularmente el TNF-α, parece influir en los niveles de anticuerpos en nuestra población de estudio, se requiere un análisis más completo para una mejor generalización debido a la naturaleza de nuestro estudio piloto.

Keywords: Antibodies; Covid-19; Cytokines; Polymorphism; Vaccines.

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Conflict of interest statement

Phey Liana, Zen Hafy, Soilia Fertilita, Ella Amalia, and Veny Larasati has received a research grant from Sriwijaya University Faculty of Medicine. The remaining author has no conflicts of interest to declare.

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