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. 1986 Jul 21;39(3):195-9.
doi: 10.1016/0024-3205(86)90530-8.

A simplified procedure for the purification of large quantities of fetal bovine serum acetylcholinesterase

D De la Hoz, B P Doctor, J S Ralston, R S Rush, A D Wolfe

A simplified procedure for the purification of large quantities of fetal bovine serum acetylcholinesterase

D De la Hoz et al. Life Sci. .

Abstract

A simple procedure has been developed for the large scale purification of fetal bovine serum acetylcholinesterase (AChE) (EC 3.1.1.7). The procedure involves two steps: batch adsorption of the AChE from 250 L of serum onto a procainamide affinity Sepharose 4B gel; and analytical procainamide affinity chromatography of the step-1 product. Over 100 mg of AChE was purified in 10 days to apparent homogeneity with this procedure.

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