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. 2023 Apr 27;85(6):2617-2627.
doi: 10.1097/MS9.0000000000000466. eCollection 2023 Jun.

Anti-inflammatory potential of 1- O-methyl chrysophanol from Amycolatopsis thermoflava ICTA 103: an exploratory study

Uma Rajeswari Batchu  1 , Joshna Rani Surapaneni  1 , Chandrasekhar Cheemalamarri  2 , Kiranmai Mandava  3 , Nagaprasad Puvvada  4 , Prakasham Reddy Shetty  2 , Surekha Mv  5
Affiliations

Anti-inflammatory potential of 1- O-methyl chrysophanol from Amycolatopsis thermoflava ICTA 103: an exploratory study

Uma Rajeswari Batchu et al. Ann Med Surg (Lond). .

Abstract

The present study was designed to investigate the anti-inflammatory potential of Amycolatopsis thermoflava producing 1-O-methyl chrysophanol (OMC), a member of the hydroxyanthraquinone family. The anti-inflammatory potential was evaluated initially through in silico analysis against tumor necrosis factor- α and cyclooxygenase-2. The same activity was further confirmed based on the in vitro protein denaturation method as well as in vivo by a carrageenan-induced paw edema model in rats. The OMC compound was isolated, purified, and characterized from the fermentation broth of Amycoloptosis thermoflava. In vitro data revealed that the OMC possesses significant protein denaturation properties with an IC50 of 63.50±2.19 µg/ml higher than the standard drug, with an IC50 value of 71.42±0.715 µg/ml. The percentage of inhibition in paw swelling was observed to be 40.03±5.5 in OMC-treated group, which is comparable to the standard group (52.8±4.7). The histopathological evaluation and immunohistochemistry revealed the anti-inflammatory potential of OMC.

Keywords: 1-O-methyl chrysophanol; amycolatopsis thermoflava; anti-inflammatory; carrageenan-induced paw edema model; molecular docking studies; protein denaturation.

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Conflict of interest statement

The authors declared that there are no conflicts of interest.

Figures

Figure 1
Figure 1
Interaction of TNF-α with OMC (A) 3D structure (B) Ligplot structure.
Figure 2
Figure 2
Interaction of TNF-α with Indomethacin (A) 3D structure (B) Ligplot structure.
Figure 3
Figure 3
Interaction of COX-2 with Indomethacin (A) 3D structure (B) Ligplot structure.
Figure 4
Figure 4
Interaction of COX-2 with OMC (A) 3D structure (B) Ligplot structure.
Figure 5
Figure 5
In vitro effect of OMC on protein denaturation.
Figure 6
Figure 6
In vivo inhibitory effect of OMC on Carrageenan-induced rat paw edema.
Figure 7
Figure 7
Histopathology of rat paw tissue (H&E stain - 10×magnification): (A) Vehicle control (B) 1% Carrageenan-induced (C) Carrageenan + indomethacin (D) Carragenan + OMC. Arrows indicate the infiltration of acute inflammatory cells.
Figure 8
Figure 8
Effect of OMC on histopathology of carrageenan-induced rat paw tissue: (A) Inflammatory cells (B) Edema. *P<0.001 level of statistical significance.
Figure 9
Figure 9
Immunohistochemical evaluation of rat paw tissue (A) CD80 (B)Arginase (C) CD 31 (D) CD68. Arrows indicate the staining area.
Figure 10
Figure 10
Effect of OMC on levels of (A) Arginase-I (B) CD80 (C) CD 31 (D) CD68 in rat paw tissue. *P<0.001 level of statistical significance.
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