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. 2023 Jun 26;13(1):10316.
doi: 10.1038/s41598-023-37507-0.

Exploring the potential of Bornean polypore fungi as biological control agents against pathogenic Ganoderma boninense causing basal stem rot in oil palm

Darwana Darlis #  1 , Mohamadu Boyie Jalloh  1 , Clament Fui Seung Chin  1 , Noor Khairani Mohamad Basri #  1 , Normah Awang Besar  2 , Khairulmazmi Ahmad  3 , Mohd Rashid Mohd Rakib #  4
Affiliations

Exploring the potential of Bornean polypore fungi as biological control agents against pathogenic Ganoderma boninense causing basal stem rot in oil palm

Darwana Darlis et al. Sci Rep. .

Abstract

Basal stem rot due to a fungal pathogen, Ganoderma boninense, is one of the most devastating diseases in oil palm throughout the major palm oil producer countries. This study investigated the potential of polypore fungi as biological control agents against pathogenic G. boninense in oil palm. In vitro antagonistic screening of selected non-pathogenic polypore fungi was performed. Based on in planta fungi inoculation on oil palm seedlings, eight of the 21 fungi isolates tested (GL01, GL01, RDC06, RDC24, SRP11, SRP12, SRP17, and SRP18) were non-pathogenic. In vitro antagonistic assays against G. boninense revealed that the percentage inhibition of radial growth (PIRG) in dual culture assay for SRP11 (69.7%), SRP17 (67.3%), and SRP18 (72.7%) was relatively high. Percentage inhibition of diameter growth (PIDG) in volatile organic compounds (VOCs) in dual plate assay of SRP11, SRP17, and SRP18 isolates were 43.2%, 51.6%, and 52.1%, respectively. Molecular identification using the internal transcribed spacer gene sequences of SRP11, SRP17, and SRP18 isolates revealed that they were Fomes sp., Trametes elegans, and Trametes lactinea, respectively.

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Conflict of interest statement

The authors declare no competing interests.

Figures

Figure 1
Figure 1
Disease signs and symptoms in oil palm seedlings at four weeks after the inoculation of polypore fungi, in planta. (a) Healthy or symptomless seedling. (b) Chlorotic (i) and necrotic (ii) leaves. (c) Fungal mass on an infected seedling (arrow). (d) Decayed primary roots (arrow). (e) Leaves of healthy (i) and infected (ii) seedlings. (f) Roots of healthy (i) and infected (ii) seedlings.
Figure 2
Figure 2
In vitro dual culture assay showing inhibition of the potential antagonists against G. boninense (G4), where the inhibition is indicated by arrows. (a) Antagonism of SRP11 against G. boninense (G4). (b) Antagonism of SRP17 against G. boninense (G4). c antagonism of SRP17 against G. boninense (G4).
Figure 3
Figure 3
Schematic diagram of the volatile organic compound dual plate assay setup. (a) Three-dimensional view of the experimental unit. (b) Side view of the experimental unit.
See this image and copyright information in PMC

References

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