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. 2023:2692:1-13.
doi: 10.1007/978-1-0716-3338-0_1.

Bacterial Binding, Phagocytosis, and Killing Capacity: Measurements Using Colony Forming Units

Kyle E Novakowski  1 , Dessi Loukov  1 , Dawn M E Bowdish  2
Affiliations

Bacterial Binding, Phagocytosis, and Killing Capacity: Measurements Using Colony Forming Units

Kyle E Novakowski et al. Methods Mol Biol. 2023.

Abstract

Herein, we provide a colony forming unit (CFU)-based counting method for quantitating the bacterial binding, phagocytosis, and killing capacity of phagocytes. Although these functions can be measured by immunofluorescence- and dye-based assays, quantitating CFUs are comparatively inexpensive and easy to perform. The protocol described below is easily modified for use with different phagocytes (e.g., macrophages, neutrophils, cell lines), types of bacteria, or opsonic conditions.

Keywords: Bacterial killing; Gentamicin protection; Macrophage; Particle binding; Phagocyte; Phagocytosis.

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