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. 2023 Jun;9(6):e17438.
doi: 10.1016/j.heliyon.2023.e17438. Epub 2023 Jun 18.

Rapid RT-PCR identification of SARS-CoV-2 in screening donors of fecal microbiota transplantation

Sara Scaglione  1 , Franca Gotta  1 , Daria Vay  1 , Christian Leli  1 , Annalisa Roveta  2 , Antonio Maconi  2 , Andrea Rocchetti  1
Affiliations

Rapid RT-PCR identification of SARS-CoV-2 in screening donors of fecal microbiota transplantation

Sara Scaglione et al. Heliyon. 2023 Jun.

Abstract

Since its first appearance in late 2019 in Wuhan, China, severe acute respiratory syndrome caused by Coronavirus 2 (SARS-CoV-2) has had a major impact on healthcare facilities around the world. Although in the past year, mass vaccination and the development of monoclonal antibody treatments have reduced the number of deaths and severe cases, the circulation of SARS-CoV-2 remains high. Over the past two years, diagnostics have played a crucial role in virus containment both in health care facilities and at the community level. For SARS-CoV-2 detection, the commonly used specimen type is the nasopharyngeal swab, although the virus can be identified in other matrices such as feces. Since fecal microbiota transplantation (FMT) assumes significant importance in the treatment of chronic gut infections and that feces may be a potential vehicle for transmission of SARS-CoV-2, in this study we have evaluated the performance of the rapid cartridge-based RT-PCR test STANDARD™ M10 SARS-CoV-2 (SD Biosensor Inc., Suwon, South Korea) using fecal samples. The results obtained indicates that STANDARD™ M10 SARS-CoV-2 can detect SARS-CoV-2 in stool samples even at low concentration. For this reason, STANDARD™ M10 SARS-CoV-2 could be used as reliable methods for the detection of SARS-CoV-2 in fecal samples and for the screening of FMT donors.

Keywords: Fecal microbiota transplantation (FMT); Performance evaluation; RT-PCR; STANDARD™ M10 SARS-CoV-2.

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Conflict of interest statement

The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

Figures

Fig. 1
Fig. 1
Distribution of CT values of target E and ORF1ab in known SARS-CoV-2 samples at various concentration in the presence and absence of fecal material. The two horizontal lines at the ends of the graph represent the highest and the lowest CT value. The ‘colored box’ is bounded by the first quartile and the third quartile. Within this range is the dark line identifying the median: the central value of the data distribution. 1A CT distribution of E gene target The differences in these values between the two experimental conditions are not statistically significant (p-value 0.8015). 1B CT distribution of ORF1ab target gene. The differences in these values between the two experimental conditions were not statistically significant (p-value 0.0767).
Fig. 2
Fig. 2
Schematic representation of the experiment conducted (A) in presence and (B) in absence of stool.
See this image and copyright information in PMC

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