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Review
. 2023 Jun 1;13(6):605.
doi: 10.3390/bios13060605.

Application of Shear Horizontal Surface Acoustic Wave (SH-SAW) Immunosensor in Point-of-Care Diagnosis

Chia-Hsuan Cheng  1   2 , Hiromi Yatsuda  1   2 , Mikihiro Goto  3 , Jun Kondoh  1 , Szu-Heng Liu  2 , Robert Y L Wang  4   5   6
Affiliations
Review

Application of Shear Horizontal Surface Acoustic Wave (SH-SAW) Immunosensor in Point-of-Care Diagnosis

Chia-Hsuan Cheng et al. Biosensors (Basel). .

Abstract

Point-of-care testing (POCT), also known as on-site or near-patient testing, has been exploding in the last 20 years. A favorable POCT device requires minimal sample handling (e.g., finger-prick samples, but plasma for analysis), minimal sample volume (e.g., one drop of blood), and very fast results. Shear horizontal surface acoustic wave (SH-SAW) biosensors have attracted a lot of attention as one of the effective solutions to complete whole blood measurements in less than 3 min, while providing a low-cost and small-sized device. This review provides an overview of the SH-SAW biosensor system that has been successfully commercialized for medical use. Three unique features of the system are a disposable test cartridge with an SH-SAW sensor chip, a mass-produced bio-coating, and a palm-sized reader. This paper first discusses the characteristics and performance of the SH-SAW sensor system. Subsequently, the method of cross-linking biomaterials and the analysis of SH-SAW real-time signals are investigated, and the detection range and detection limit are presented.

Keywords: C-reactive protein; POCT; SAW; SH-SAW; apolipoprotein; lipoprotein; point-of-care testing; simulation; surface acoustic wave; whole blood measurement.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
SH-SAW biosensor chip with POCT platform.
Figure 2
Figure 2
Phase change due to SH-SAW velocity change.
Figure 3
Figure 3
The model structures of simulation program. (a) the substrate layer (I) is 36Y-90X quartz, the metal layer (II) is gold, the additional material layer (III) is the protein layer and the liquid layer (IV) is water or a buffer; (b) The substrate layer (I) is 36Y-90X quartz, the metal layer (II) is gold and the liquid layer (III) is a glycerol-water mixture.
Figure 4
Figure 4
False grating area between the FEUDT and the sensing area.
Figure 5
Figure 5
Signal analysis from SH-SAW.
Figure 6
Figure 6
Multiple step measurement of CRP biosensor.
See this image and copyright information in PMC

References

    1. Piovarci I., Melikishvili S., Tatarko M., Hianik T., Thompson M. Detection of Sub-Nanomolar Concentration of Trypsin by Thickness-Shear Mode Acoustic Biosensor and Spectrophotometry. Biosensors. 2021;11:117. doi: 10.3390/bios11040117. - DOI - PMC - PubMed
    1. Liu J., Chen D., Wang P., Song G., Zhang X., Li Z., Wang Y., Wang J., Yang J. A microfabricated thickness shear mode electroacoustic resonator for the label-free detection of cardiac troponin in serum. Talanta. 2020;215:120890. doi: 10.1016/j.talanta.2020.120890. - DOI - PubMed
    1. Wu H., Zhao G., Zu H., Wang J.H., Wang Q.M. Real-Time Monitoring of Platelet Activation Using Quartz Thickness-Shear Mode Resonator Sensors. Biophys. J. 2016;110:669–679. doi: 10.1016/j.bpj.2015.11.3511. - DOI - PMC - PubMed
    1. Dahint R., Bender F., Morhard F. Operation of acoustic plate mode immunosensors in complex biological media. Anal. Chem. 1999;71:3150–3156. doi: 10.1021/ac990119u. - DOI - PubMed
    1. Huang Y., Das P.K., Bhethanabotla V.R. Surface acoustic waves in biosensing applications. Sens. Actuators Rep. 2021;3:100041. doi: 10.1016/j.snr.2021.100041. - DOI