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. 2023 Jun 2;21(6):344.
doi: 10.3390/md21060344.

Pentaketides and 5- p-Hydroxyphenyl-2-pyridone Derivative from the Culture Extract of a Marine Sponge-Associated Fungus Hamigera avellanea KUFA0732

Rotchana Klaram  1   2 , Tida Dethoup  2 , Fátima P Machado  3 , Luís Gales  1   4 , Decha Kumla  5 , Salar Hafez Ghoran  6 , Emília Sousa  7 , Sharad Mistry  8 , Artur M S Silva  9 , Anake Kijjoa  1   3
Affiliations

Pentaketides and 5- p-Hydroxyphenyl-2-pyridone Derivative from the Culture Extract of a Marine Sponge-Associated Fungus Hamigera avellanea KUFA0732

Rotchana Klaram et al. Mar Drugs. .

Abstract

Five undescribed pentaketide derivatives, (R)-6,8-dihydroxy-4,5-dimethyl-3-methylidene-3,4-dihydro-1H-2-benzopyran-1-one (1), [(3S,4R)-3,8-dihydroxy-6-methoxy-4,5-dimethyl-1-oxo-3,4-dihydro-1H-isochromen-3-yl]methyl acetate (2), (R)-5, 7-dimethoxy-3-((S)-(1-hydroxyethyl)-3,4-dimethylisobenzofuran-1(3H)-one (4b), (S)-7-hydroxy-3-((S)-1-hydroxyethyl)-5-methoxy-3,4-dimethylisobenzofuran 1(3H)-one (5), and a p-hydroxyphenyl-2-pyridone derivative, avellaneanone (6), were isolated together with the previously reported (R)-3-acetyl-7-hydroxy-5-methoxy-3,4-dimethylisobenzofuran-1(3H)-one (3), (R)-7-hydroxy-3-((S)-1-hydroxyethyl)-5-methoxy-3,4-dimethylisobenzofuran-1(3H)-one (4a) and isosclerone (7), from the ethyl acetate extract of a culture of a marine sponge-derived fungus, Hamigera avellanea KUFA0732. The structures of the undescribed compounds were elucidated using 1D and 2D NMR, as well as high-resolution mass spectral analyses. The absolute configurations of the stereogenic carbons in 1, 4b, 5, and 6 were established by X-ray crystallographic analysis. The absolute configurations of C-3 and C-4 in 2 were determined by ROESY correlations and on the basis of their common biosynthetic origin with 1. The crude fungal extract and the isolated compounds 1, 3, 4b, 5, 6, and 7 were assayed for their growth inhibitory activity against various plant pathogenic fungi viz. Alternaria brassicicola, Bipolaris oryzae, Colletotrichum capsici, C. gloeosporiodes, Curvularia oryzae, Fusarium semitectum, Lasiodiplodia theobromae, Phytophthora palmivora, Pyricularia oryzae, Rhizoctonia oryzae and Sclerotium rolfsii.

Keywords: 5-p-hydroxy-2-pyridone; Aspergillaceae; Hamigera avellanea; anti-plant pathogenic fungal activity; dihydrochromone; marine sponge-associated fungus; pentaketides.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Structures of 1–7.
Figure 2
Figure 2
Key COSY (bold line) and HMBC (arrow) correlations in 1.
Figure 3
Figure 3
Ortep view of 1.
Figure 4
Figure 4
Key COSY (bold line) and HMBC (arrow) correlations in 2.
Figure 5
Figure 5
Common biosynthetic pathways of 1 and 2.
Figure 6
Figure 6
Ortep diagram of 4b.
Figure 7
Figure 7
Ortep diagram of 5.
Figure 8
Figure 8
Proposed biosynthetic pathways for 3, 4a, 4b, and 5.
Figure 9
Figure 9
Key COSY (bold lines) and HMBC (arrow) correlations in 6.
Figure 10
Figure 10
Ortep view of 6.
Figure 11
Figure 11
Proposed biosynthetic pathways of 6.
See this image and copyright information in PMC

References

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Supplementary concepts

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