Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2023 Dec;47(6):2841-2852.
doi: 10.1007/s00266-023-03450-8. Epub 2023 Jun 27.

Combined Pulsed Magnetic Field and Radiofrequency Electromagnetic Field Enhances MMP-9, Collagen-4, VEGF Synthesis to Improve Wound Healing Via Hif-1α/eNOS Pathway

Halil Asci  1   2 , Mehtap Savran  3   4 , Selcuk Comlekci  4   5 , Mehmet M Sofu  4 , Yalcin Erzurumlu  4   6 , Ozlem Ozmen  7 , Mine Kaynak  3 , Mehmet E Sahin  8 , Rumeysa Taner  4 , Mustafa Gecin  5
Affiliations

Combined Pulsed Magnetic Field and Radiofrequency Electromagnetic Field Enhances MMP-9, Collagen-4, VEGF Synthesis to Improve Wound Healing Via Hif-1α/eNOS Pathway

Halil Asci et al. Aesthetic Plast Surg. 2023 Dec.

Abstract

Background: The blood supply of the tissue is very important in the acceleration of wound healing. Radiofrequency electromagnetic field (RF) and the pulsed magnetic field (PMF) increase vasodilation to contribute wound healing. The aim of this study was to evaluate the effects of RF and PMF on wound healing via hypoxia-inducible factor-1 alpha (Hif-1α)/endothelial nitric oxide synthase (eNOS) pathway.

Methods: Forty-eight rats were divided into 4 groups as sham (wound created only), PMF (27.12 MHz, 12 times a day at 30-min intervals), RF (0.5 mT, continuously) and PMF + RF groups. Wounds were created at 1.5 × 1.5 cm size to the dorsal region, and animals were put into unit. Six animals were killed on days 4 and 7; wound tissues were collected for histopathological, immunohistochemical as collagen-4, cytokeratin, matrix metalloproteinase-9 (MMP-9), vascular endothelial growth factor (VEGF) staining and Hif-1α/eNOS/VEGF expressions.

Results: On day 4, in addition to increasing VEGF and MMP-9 stainings, connection between intact tissue and scar tissue which was stronger in the RF- and PMF-applied groups was observed. On day 7, epithelization started; inflammatory reaction decreased; collagen production, cytokeratin, VEGF and MMP-9 expression enhanced, especially in the RF + PMF applied group. eNOS, Hif-1α and VEGF expression levels were found to be significantly highest in both days of RF + PMF-applied group.

Conclusions: This study revealed that both in vitro RF and PMF applications can cause notable changes in factors that are required for tissue repair on wound healing such as epithelization, connective tissue formation, collagen production and angiogenesis via vasodilatory Hif-1α/eNOS pathway and VEGF signaling.

No level assigned: This journal requires that authors assign a level of evidence to each submission to which Evidence-Based Medicine rankings are applicable. This excludes Review Articles, Book Reviews, and manuscripts that concern Basic Science, Animal Studies, Cadaver Studies, and Experimental Studies. For a full description of these Evidence-Based Medicine ratings, please refer to the Table of Contents or the online Instructions to Authors www.springer.com/00266 .

Keywords: Hif-1α; Pulsed magnetic field; Radiofrequency electromagnetic field; Wound healing; eNOS.

PubMed Disclaimer

References

    1. Wilkinson HN, Hardman MJ (2020) Wound healing: cellular mechanisms and pathological outcomes. Open Biol 10:200–223 - DOI
    1. Yasukawa K, Okuno T, Yokomizo T (2020) Eicosanoids in skin wound healing. Int J Mol Sci 21:8435 - DOI - PubMed - PMC
    1. Sivrice ME, Yasan H, Karahan N, Coban İ, Yetis A (2017) Effects of allantoin on wound healing in nasal mucosa. KBB-Forum 16(1):1–6
    1. Sarataş B, Tüz M, Sivrice ME, Yasan H, Bozkurt KK (2019) Effects of platelet-rich plasma on wound healing in nasal mucosa. Med J Suleyman Demirel Univ 26(4):408–411
    1. Schultz GS, Chin GA, Moldawer L, Diegelmann RF (2011) Principles of wound healing. In: Fitridge R, Thompson M (eds) Mechanisms of vascular disease: a reference book for vascular specialists. University of Adelaide Press, Adelaide (AU), p 23

LinkOut - more resources