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. 1986 Jul 11;14(13):5241-54.
doi: 10.1093/nar/14.13.5241.

Antibodies to hnRNP core proteins inhibit in vitro splicing of human beta-globin pre-mRNA

H Sierakowska, W Szer, P J Furdon, R Kole
Free PMC article

Antibodies to hnRNP core proteins inhibit in vitro splicing of human beta-globin pre-mRNA

H Sierakowska et al. Nucleic Acids Res. .
Free PMC article

Abstract

In vitro splicing of human beta-globin pre-mRNA can be fully inhibited by treatment of the splicing extract with polyclonal antibodies against hnRNP core proteins prior to the addition of pre-mRNA. Inhibition of the first step in the splicing pathway, cleavage at the 5' splice site and lariat formation, requires more antibodies than inhibition of the second step, cleavage at the 3' splice site and exon ligation. The anti-hnRNP antibodies can also inhibit the splicing reaction after the formation of the active nucleoprotein splicing complex which is known to occur during the initial lag period. Thus, hnRNP core proteins appear to be present in the complex that performs pre-mRNA splicing.

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