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. 2023 May 27;15(6):1261.
doi: 10.3390/v15061261.

Novel Amplicon-Based Sequencing Approach to West Nile Virus

Moussa Moïse Diagne  1 , Marie Henriette Dior Ndione  1 , Giulia Mencattelli  2   3   4 , Amadou Diallo  5 , El Hadji Ndiaye  6 , Marco Di Domenico  2 , Diawo Diallo  6 , Mouhamed Kane  1 , Valentina Curini  2 , Ndeye Marieme Top  5 , Maurilia Marcacci  2 , Maïmouna Mbanne  1 , Massimo Ancora  2 , Barbara Secondini  2 , Valeria Di Lollo  2 , Liana Teodori  2 , Alessandra Leone  2 , Ilaria Puglia  2 , Alioune Gaye  6 , Amadou Alpha Sall  1 , Cheikh Loucoubar  5 , Roberto Rosà  3 , Mawlouth Diallo  6 , Federica Monaco  2 , Ousmane Faye  1 , Cesare Cammà  2 , Annapaola Rizzoli  4 , Giovanni Savini  2 , Oumar Faye  1
Affiliations

Novel Amplicon-Based Sequencing Approach to West Nile Virus

Moussa Moïse Diagne et al. Viruses. .

Abstract

West Nile virus is a re-emerging arbovirus whose impact on public health is increasingly important as more and more epidemics and epizootics occur, particularly in America and Europe, with evidence of active circulation in Africa. Because birds constitute the main reservoirs, migratory movements allow the diffusion of various lineages in the world. It is therefore crucial to properly control the dispersion of these lineages, especially because some have a greater health impact on public health than others. This work describes the development and validation of a novel whole-genome amplicon-based sequencing approach to West Nile virus. This study was carried out on different strains from lineage 1 and 2 from Senegal and Italy. The presented protocol/approach showed good coverage using samples derived from several vertebrate hosts and may be valuable for West Nile genomic surveillance.

Keywords: West Nile virus; amplicon-based sequencing; lineages; next-generation sequencing.

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Conflict of interest statement

The authors declare no conflict of interest. All authors have read and agreed to the published version of the manuscript.

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