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. 2023 May 29;15(6):1270.
doi: 10.3390/v15061270.

SARS-CoV-2 Detection and Culture in Different Biological Specimens from Immunocompetent and Immunosuppressed COVID-19 Patients Infected with Two Different Viral Strains

Maria Cássia Mendes-Correa  1   2   3 , Matias Chiarastelli Salomão  1   2   4 , Fábio Ghilardi  1   3 , Tania Regina Tozetto-Mendoza  1   3 , Lucy Santos Villas-Boas  1   3 , Anderson Vicente de Paula  1   3 , Heuder Gustavo Oliveira Paiao  1   3 , Antonio Charlys da Costa  3 , Fábio E Leal  5   6 , Andrea de Barros Coscelli Ferraz  5 , Flavia C S Sales  1   3 , Ingra M Claro  1   3 , Noely E Ferreira  1   3 , Geovana M Pereira  1   3 , Almir Ribeiro da Silva Jr  1   3 , Wilton Freire  1   3 , Evelyn Patricia Sánchez Espinoza  1   3 , Erika R Manuli  1   3   5 , Camila M Romano  2   3 , Jaqueline G de Jesus  3 , Ester C Sabino  1   3   5 , Steven S Witkin  3   7
Affiliations

SARS-CoV-2 Detection and Culture in Different Biological Specimens from Immunocompetent and Immunosuppressed COVID-19 Patients Infected with Two Different Viral Strains

Maria Cássia Mendes-Correa et al. Viruses. .

Abstract

Introduction-The dynamics of SARS-CoV-2 shedding and replication in humans remain incompletely understood. Methods-We analyzed SARS-CoV-2 shedding from multiple sites in individuals with an acute COVID-19 infection by weekly sampling for five weeks in 98 immunocompetent and 25 immunosuppressed individuals. Samples and culture supernatants were tested via RT-PCR for SARS-CoV-2 to determine viral clearance rates and in vitro replication. Results-A total of 2447 clinical specimens were evaluated, including 557 nasopharyngeal swabs, 527 saliva samples, 464 urine specimens, 437 anal swabs and 462 blood samples. The SARS-CoV-2 genome sequences at each site were classified as belonging to the B.1.128 (ancestral strain) or Gamma lineage. SARS-CoV-2 detection was highest in nasopharyngeal swabs regardless of the virus strain involved or the immune status of infected individuals. The duration of viral shedding varied between clinical specimens and individual patients. Prolonged shedding of potentially infectious virus varied from 10 days up to 191 days, and primarily occurred in immunosuppressed individuals. Virus was isolated in culture from 18 nasal swab or saliva samples collected 10 or more days after onset of disease. Conclusions-Our findings indicate that persistent SARS-CoV-2 shedding may occur in both competent or immunosuppressed individuals, at multiple clinical sites and in a minority of subjects is capable of in vitro replication.

Keywords: COVID-19; SARS-CoV-2; cell culture; infectiousness; persistence; viral load dynamics; viral shedding.

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Conflict of interest statement

The authors declare no conflict of interest.

References

    1. Puhach O., Meyer B., Eckerle I. SARS-CoV-2 viral load and shedding kinetics. Nat. Rev. Microbiol. 2023;21:147–161. doi: 10.1038/s41579-022-00822-w. - DOI - PMC - PubMed
    1. Néant N., Lingas G., Le Hingrat Q., Ghosn J., Engelmann I., Lepiller Q., Gaymard A., Ferré V., Hartard C., Plantier J.C., et al. French COVID Cohort Investigators and French Cohort Study groups. Modeling SARS-CoV-2 viral kinetics and association with mortality in hospitalized patients from the French COVID cohort. Proc. Natl. Acad. Sci. USA. 2021;118:e2017962118. doi: 10.1073/pnas.2017962118. - DOI - PMC - PubMed
    1. Fajnzylber J., Regan J., Coxen K., Corry H., Wong C., Rosenthal A., Worrall D., Giguel F., Piechocka-Trocha A., Atyeo C., et al. Massachusetts consortium for pathogen readiness. SARS-CoV-2 viral load is associated with increased disease severity and mortality. Nat. Commun. 2020;11:5493. doi: 10.1038/s41467-020-19057-5. - DOI - PMC - PubMed
    1. Zheng S., Fan J., Yu F., Feng B., Lou B., Zou Q., Xie G., Lin S., Wang R., Yang X., et al. Viral load dynamics and disease severity in patients infected with SARS-CoV-2 in Zhejiang province, China, January–March 2020: Retrospective cohort study. BMJ. 2020;369:m1443. doi: 10.1136/bmj.m1443. - DOI - PMC - PubMed
    1. Cevik M., Tate M., Lloyd O., Maraolo A.E., Schafers J., Ho A. SARS-CoV-2, SARS-CoV, and MERS-CoV viral load dynamics, duration of viral shedding, and infectiousness: A systematic review and meta-analysis. Lancet Microbe. 2021;2:e13–e22. doi: 10.1016/S2666-5247(20)30172-5. - DOI - PMC - PubMed

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