Standard: Human intestinal cancer organoids

Hanqing Lin^#¹, Yalong Wang^#^{2

3

4

5}, Chunyan Cheng^#¹, Yuxin Qian^#¹, Jie Hao^{6

7

8

9}, Zhen Zhang^{10

11

12}, Weiqi Sheng^{11

13}, Linhong Song¹⁴, Chu-Xia Deng¹⁵, Bing Zhao¹⁶, Jiani Cao^{6

7

8

9}, Lei Wang^{6

7

8

9}, Liu Wang^{6

7

8

9}, Lingmin Liang^{6

7

8

9}, Wenli Kelly Chen¹⁷, Chunping Yu¹⁸, Zhijian Sun¹⁹, Yingying Yang²⁰, Changlin Wang^{9

21}, Yong Zhang^{22

23}, Qiyuan Li^{9

24}, Ka Li^{9

22}, Aijin Ma^{25

26}, Tongbiao Zhao^{27

28

29

30}, Ye-Guang Chen^{31

32

33}, Guoqiang Hua^{34

35}

Affiliations

¹ D1Med Technology (Shanghai) Inc, Shanghai, 201802, China.
² The State Key Laboratory of Membrane Biology, Tsinghua-Peking Center for Life Sciences, School of Life Sciences, Tsinghua University, Beijing, 100084, China.
³ Guangzhou Laboratory, Guangzhou, 510005, China.
⁴ Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, 510530, China.
⁵ Guangzhou Hua Yi Regeneration Technology Co., Ltd, Huangpu District, Guangzhou, 510700, China.
⁶ National Stem Cell Resource Center, State Key Laboratory of Stem Cell and Reproductive Biology, Institute for Stem Cell and Regeneration, Institute of Zoology, Chinese Academy of Sciences, Beijing, 100101, China.
⁷ Beijing Institute for Stem Cell and Regenerative Medicine, Beijing, 100101, China.
⁸ University of Chinese Academy of Sciences, Beijing, 100049, China.
⁹ Institute of Clinical Science, Zhongshan Hospital, Fudan University, Shanghai, 200433, China.
¹⁰ Department of Radiation Oncology and Cancer Institute, Fudan University Shanghai Cancer Center Fudan University, Shanghai, 200032, China.
¹¹ Department of Oncology, Shanghai Medical College, Fudan University, Shanghai, 200032, China.
¹² Shanghai Key Laboratory of Radiation Oncology, Shanghai, 200032, China.
¹³ Department of Pathology, Fudan University Shanghai Cancer Center, Shanghai, 200032, China.
¹⁴ Sichuan Academy of Medical Sciences & Sichuan Provincial People's Hospital, Chengdu, 610072, China.
¹⁵ Cancer Centre, Faculty of Health Sciences, University of Macau, Macau, 999078, SAR, China.
¹⁶ State Key Laboratory of Genetic Engineering, School of Life Sciences, Fudan University, Shanghai, 200438, China.
¹⁷ China Innovation Center of Roche, Li Shi Zhen Road, Pudong, Shanghai, 201203, China.
¹⁸ Eli Lilly and Company, Pudong, Shanghai, 201203, China.
¹⁹ K2 Oncology Co., Ltd, KeChuang Street, Beijing, 100176, China.
²⁰ Qilu Pharmaceutical Co., Ltd, Jinan, 250104, China.
²¹ China National Institute of Standardization, Beijing, 100191, China.
²² Chinese Society for Stem Cell Research, Shanghai, 200032, China.
²³ HHLIFE Co., Inc, Shenzhen, 518040, China.
²⁴ China National GeneBank, Shenzhen, 518000, China.
²⁵ Institute of Clinical Science, Zhongshan Hospital, Fudan University, Shanghai, 200433, China. 783869855@qq.com.
²⁶ Beijing Technology and Business University, Beijing, 100048, China. 783869855@qq.com.
²⁷ National Stem Cell Resource Center, State Key Laboratory of Stem Cell and Reproductive Biology, Institute for Stem Cell and Regeneration, Institute of Zoology, Chinese Academy of Sciences, Beijing, 100101, China. tbzhao@ioz.ac.cn.
²⁸ Beijing Institute for Stem Cell and Regenerative Medicine, Beijing, 100101, China. tbzhao@ioz.ac.cn.
²⁹ University of Chinese Academy of Sciences, Beijing, 100049, China. tbzhao@ioz.ac.cn.
³⁰ Institute of Clinical Science, Zhongshan Hospital, Fudan University, Shanghai, 200433, China. tbzhao@ioz.ac.cn.
³¹ The State Key Laboratory of Membrane Biology, Tsinghua-Peking Center for Life Sciences, School of Life Sciences, Tsinghua University, Beijing, 100084, China. ygchen@tsinghua.edu.cn.
³² Guangzhou Laboratory, Guangzhou, 510005, China. ygchen@tsinghua.edu.cn.
³³ School of Basic Medicine, Jiangxi Medical College, Nanchang University, Nanchang, 330031, China. ygchen@tsinghua.edu.cn.
³⁴ D1Med Technology (Shanghai) Inc, Shanghai, 201802, China. guoqianghua@fudan.edu.cn.
³⁵ Department of Radiation Oncology and Cancer Institute, Fudan University Shanghai Cancer Center Fudan University, Shanghai, 200032, China. guoqianghua@fudan.edu.cn.

^# Contributed equally.

PMID: 37378693
PMCID: PMC10307733
DOI: 10.1186/s13619-023-00167-6

Editorial

Standard: Human intestinal cancer organoids

Hanqing Lin et al. Cell Regen. 2023.

. 2023 Jun 28;12(1):24.

doi: 10.1186/s13619-023-00167-6.

Authors

Affiliations

¹ D1Med Technology (Shanghai) Inc, Shanghai, 201802, China.
² The State Key Laboratory of Membrane Biology, Tsinghua-Peking Center for Life Sciences, School of Life Sciences, Tsinghua University, Beijing, 100084, China.
³ Guangzhou Laboratory, Guangzhou, 510005, China.
⁴ Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, 510530, China.
⁵ Guangzhou Hua Yi Regeneration Technology Co., Ltd, Huangpu District, Guangzhou, 510700, China.
⁶ National Stem Cell Resource Center, State Key Laboratory of Stem Cell and Reproductive Biology, Institute for Stem Cell and Regeneration, Institute of Zoology, Chinese Academy of Sciences, Beijing, 100101, China.
⁷ Beijing Institute for Stem Cell and Regenerative Medicine, Beijing, 100101, China.
⁸ University of Chinese Academy of Sciences, Beijing, 100049, China.
⁹ Institute of Clinical Science, Zhongshan Hospital, Fudan University, Shanghai, 200433, China.
¹⁰ Department of Radiation Oncology and Cancer Institute, Fudan University Shanghai Cancer Center Fudan University, Shanghai, 200032, China.
¹¹ Department of Oncology, Shanghai Medical College, Fudan University, Shanghai, 200032, China.
¹² Shanghai Key Laboratory of Radiation Oncology, Shanghai, 200032, China.
¹³ Department of Pathology, Fudan University Shanghai Cancer Center, Shanghai, 200032, China.
¹⁴ Sichuan Academy of Medical Sciences & Sichuan Provincial People's Hospital, Chengdu, 610072, China.
¹⁵ Cancer Centre, Faculty of Health Sciences, University of Macau, Macau, 999078, SAR, China.
¹⁶ State Key Laboratory of Genetic Engineering, School of Life Sciences, Fudan University, Shanghai, 200438, China.
¹⁷ China Innovation Center of Roche, Li Shi Zhen Road, Pudong, Shanghai, 201203, China.
¹⁸ Eli Lilly and Company, Pudong, Shanghai, 201203, China.
¹⁹ K2 Oncology Co., Ltd, KeChuang Street, Beijing, 100176, China.
²⁰ Qilu Pharmaceutical Co., Ltd, Jinan, 250104, China.
²¹ China National Institute of Standardization, Beijing, 100191, China.
²² Chinese Society for Stem Cell Research, Shanghai, 200032, China.
²³ HHLIFE Co., Inc, Shenzhen, 518040, China.
²⁴ China National GeneBank, Shenzhen, 518000, China.
²⁵ Institute of Clinical Science, Zhongshan Hospital, Fudan University, Shanghai, 200433, China. 783869855@qq.com.
²⁶ Beijing Technology and Business University, Beijing, 100048, China. 783869855@qq.com.
²⁷ National Stem Cell Resource Center, State Key Laboratory of Stem Cell and Reproductive Biology, Institute for Stem Cell and Regeneration, Institute of Zoology, Chinese Academy of Sciences, Beijing, 100101, China. tbzhao@ioz.ac.cn.
²⁸ Beijing Institute for Stem Cell and Regenerative Medicine, Beijing, 100101, China. tbzhao@ioz.ac.cn.
²⁹ University of Chinese Academy of Sciences, Beijing, 100049, China. tbzhao@ioz.ac.cn.
³⁰ Institute of Clinical Science, Zhongshan Hospital, Fudan University, Shanghai, 200433, China. tbzhao@ioz.ac.cn.
³¹ The State Key Laboratory of Membrane Biology, Tsinghua-Peking Center for Life Sciences, School of Life Sciences, Tsinghua University, Beijing, 100084, China. ygchen@tsinghua.edu.cn.
³² Guangzhou Laboratory, Guangzhou, 510005, China. ygchen@tsinghua.edu.cn.
³³ School of Basic Medicine, Jiangxi Medical College, Nanchang University, Nanchang, 330031, China. ygchen@tsinghua.edu.cn.
³⁴ D1Med Technology (Shanghai) Inc, Shanghai, 201802, China. guoqianghua@fudan.edu.cn.
³⁵ Department of Radiation Oncology and Cancer Institute, Fudan University Shanghai Cancer Center Fudan University, Shanghai, 200032, China. guoqianghua@fudan.edu.cn.

^# Contributed equally.

PMID: 37378693
PMCID: PMC10307733
DOI: 10.1186/s13619-023-00167-6

Abstract

Intestinal cancer is one of the most frequent and lethal types of cancer. Modeling intestinal cancer using organoids has emerged in the last decade. Human intestinal cancer organoids are physiologically relevant in vitro models, which provides an unprecedented opportunity for fundamental and applied research in colorectal cancer. "Human intestinal cancer organoids" is the first set of guidelines on human intestinal organoids in China, jointly drafted and agreed by the experts from the Chinese Society for Cell Biology and its branch society: the Chinese Society for Stem Cell Research. This standard specifies terms and definitions, technical requirements, test methods for human intestinal cancer organoids, which apply to the production and quality control during the process of manufacturing and testing of human intestinal cancer organoids. It was released by the Chinese Society for Cell Biology on 24 September 2022. We hope that the publication of this standard will guide institutional establishment, acceptance and execution of proper practocal protocols, and accelerate the international standardization of human intestinal cancer organoids for clinical development and therapeutic applications.

PubMed Disclaimer

Conflict of interest statement

The authors declare no competing financial interests. Y.-G.C. is the Editor‑in‑Chief of Cell Regeneration. He was not involved in the review or decision related to this manuscript. This work was not sponsored by any commercial organizations, and all the other authors declare that they have no competing interests.

References

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Standard: Human intestinal cancer organoids

Affiliations

Standard: Human intestinal cancer organoids

Authors

Affiliations

Abstract

Conflict of interest statement

References

Publication types

LinkOut - more resources

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