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Comment
. 2023 Aug 24;8(4):e0027123.
doi: 10.1128/msphere.00271-23. Epub 2023 Jun 29.

mSphere of Influence: Systemic decoding gene function in Toxoplasma gondii pathogenesis-CRISPR screens and beyond

Yifan Wang  1
Affiliations
Comment

mSphere of Influence: Systemic decoding gene function in Toxoplasma gondii pathogenesis-CRISPR screens and beyond

Yifan Wang. mSphere. .

Abstract

Yifan Wang works in the field of molecular parasitology with a focus on host-pathogen interactions. In this mSphere of Influence article, he reflects on how papers entitled "A genome-wide CRISPR screen in Toxoplasma identifies essential apicomplexan genes" by S. M. Sidik, D. Huet, S. M. Ganesan, M.-H. Huynh, et al. (Cell 166:1423.e12-1435.e12, 2016, https://doi.org/10.1016/j.cell.2016.08.019) and "Mapping host-microbe transcriptional interactions by dual Perturb-seq" by S. Butterworth, K. Kordova, S. Chandrasekaran, K. K. Thomas, et al. (bioRxiv, https://doi.org/10.1101/2023.04.21.537779) made an impact on his research and changed the way he thinks how functional genomics and high-throughput screens provide novel insights into pathogen pathogenesis.

Keywords: CRISPR screen; Toxoplasma gondii; functional genomics.

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Conflict of interest statement

The author declares no conflict of interest.

Comment on

References

    1. Montoya JG, Liesenfeld O. 2004. Toxoplasmosis. Lancet 363:1965–1976. doi:10.1016/S0140-6736(04)16412-X - DOI - PubMed
    1. Kim K, Weiss LM. 2004. Toxoplasma gondii: the model apicomplexan. Int J Parasitol 34:423–432. doi:10.1016/j.ijpara.2003.12.009 - DOI - PMC - PubMed
    1. Sidik SM, Huet D, Ganesan SM, Huynh M-H, Wang T, Nasamu AS, Thiru P, Saeij JPJ, Carruthers VB, Niles JC, Lourido S. 2016. A genome-wide CRISPR screen in Toxoplasma identifies essential apicomplexan genes. Cell 166:1423–1435. doi:10.1016/j.cell.2016.08.019 - DOI - PMC - PubMed
    1. Butterworth S, Kordova K, Chandrasekaran S, Thomas KK, Torelli F, Lockyer EJ, Edwards A, Goldstone R, Koshy AA, Treeck M. 2023. Mapping host-microbe transcriptional interactions by dual perturb-seq. bioRxiv. doi:10.1101/2023.04.21.537779 - DOI - PubMed
    1. Wang Y, Sangaré LO, Paredes-Santos TC, Hassan MA, Krishnamurthy S, Furuta AM, Markus BM, Lourido S, Saeij JPJ. 2020. Genome-wide screens identify Toxoplasma gondii determinants of parasite fitness in IFNγ-activated murine macrophages. Nat Commun 11:5258. doi:10.1038/s41467-020-18991-8 - DOI - PMC - PubMed

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