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. 2023 Nov:211:106328.
doi: 10.1016/j.pep.2023.106328. Epub 2023 Jun 29.

Robust production of active Ulp1 (SUMO protease) from inclusion bodies

Bala Kota Reddy Patakottu  1 , Vikram Reddy Vedire  2 , Chereddy Ramamohan Reddy  2
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Free article

Robust production of active Ulp1 (SUMO protease) from inclusion bodies

Bala Kota Reddy Patakottu et al. Protein Expr Purif. 2023 Nov.
Free article

Abstract

High yield purification of Ulp1 is required during the isolation and purification of SUMO-tagged recombinant proteins. However, when expressed as a soluble protein, Ulp1 is toxic to E. coli host cells and most of the protein forms inclusion bodies. The extraction of insoluble Ulp1 followed by its purification and refolding into its active form is a lengthy and costly procedure. In our present study, we developed a simple, cost effective procedure for the large scale production of active Ulp1 that can be used for industrial scale requirements.

Keywords: E. coli BL21(DE3); SUMO (small ubiquitin like modifier protein); Ulp1.

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Conflict of interest statement

Declaration of competing interest BKRP, VRV and CRR are stock holders of Loka Biosciences Pvt. Ltd. BKRP is the director of operations; VRV and CRR are the managing directors. All the authors declare that they have no conflict of interest.

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