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. 2023 Jul 12;21(27):5648-5657.
doi: 10.1039/d2ob02181a.

Photo-induced crosslinking uncovers an antiparallel strand orientation in heterodimeric (EIAALEK)3/(KIAALKE)3 and (EIAALEK)3/(RIAALRE)3 coiled-coil systems

D Aerssens  1 , L Miret-Casals  1 , D Gomez  2 , D Sousa-Neves  1 , Y Levy  3 , M De Vleesschouwer  1 , A Manicardi  1   4 , A Madder  1
Affiliations

Photo-induced crosslinking uncovers an antiparallel strand orientation in heterodimeric (EIAALEK)3/(KIAALKE)3 and (EIAALEK)3/(RIAALRE)3 coiled-coil systems

D Aerssens et al. Org Biomol Chem. .

Abstract

We describe for the first time the co-existence of the parallel and antiparallel conformation of the heterodimeric E3/K3 and E3/R3 coiled-coil systems in solution. The introduction of a furanylated amino acid in the (EIAALEK)3 sequence allowed, upon photo-induced covalent crosslinking, freezing of the respective coiled-coil complexes present in solution. The occurrence of both parallel and antiparallel conformations in solution was supported by computational simulations and further confirmed by fluorescence experiments based on pyrene-pyrene stacking.

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