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. 2023 May 9:18:100705.
doi: 10.1016/j.fochx.2023.100705. eCollection 2023 Jun 30.

Comparative analysis of oligosaccharides in the milk of human and animals by using LC-QE-HF-MS

Rui Li  1   2   3 , Yalin Zhou  1   2 , Yajun Xu  1   2   3
Affiliations

Comparative analysis of oligosaccharides in the milk of human and animals by using LC-QE-HF-MS

Rui Li et al. Food Chem X. .

Abstract

The complex oligosaccharides (OS) in different milk are more difficult to detect and complicated to analyze as their enormous structural complexity. UPLC-QE-HF-MS was supposed to be a highly effective method for OS identification. In present study, 70 human milk oligosaccharides (HMOs), 14 bovine milk oligosaccharides (BMOs), 23 goat milk oligosaccharides (GMOs) and 24 rat milk oligosaccharides (RMOs) were detected by using UPLC-QE-HF-MS, respectively. There were highly differences in number and composition between the four milk OS. 14 neutral and 3 acidic OS were firstly found in rat milk. The composition and abundances of RMOs were might more similar to that of HMOs, comparing with BMOs and GMOs. The similarity between HMOs and RMOs might provide theoretical basis for better application of rats in biological/biomedical studies of HMOs as models. The BMOs and GMOs were expected to be suitable for applications in medical and functional foods as a promising bioactive molecular.

Keywords: Composition; Identification; Liquid chromatography-Q exactive-HF hybrid quadrupole-Orbitrap-mass spectrometry; Milk oligosaccharides.

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Conflict of interest statement

The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

Figures

Fig. 1
Fig. 1
Total ion chromatogram of OS from (A1&A2) Human milk, (B1&B2) Bovine milk, (C1&C2) Goat milk and (D1&D2) Rat milk.
Fig. 2
Fig. 2
The abundance (peak area) (A) and relative content (B) of HexNAc and fucosylated OS in various milk.
Fig. 3
Fig. 3
The abundance (peak area) (A) and relative content (B) of NeuAc, NeuGc and NeuAcNeuGc OS in various milk.
See this image and copyright information in PMC

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