Minimum information guidelines for experiments structurally characterizing intrinsically disordered protein regions

Bálint Mészáros^{1

2}, András Hatos^#^{3

4

5

6

7}, Nicolas Palopoli^#⁸, Federica Quaglia^#^{3

9}, Edoardo Salladini^#³, Kim Van Roey^#^{10

11}, Haribabu Arthanari^{12

13}, Zsuzsanna Dosztányi¹⁴, Isabella C Felli¹⁵, Patrick D Fischer^{12

13}, Jeffrey C Hoch¹⁶, Cy M Jeffries¹⁷, Sonia Longhi¹⁸, Emiliano Maiani^{19

20}, Sandra Orchard²¹, Rita Pancsa²², Elena Papaleo^{19

23}, Roberta Pierattelli¹⁵, Damiano Piovesan³, Iva Pritisanac^{24

25}, Luiggi Tenorio³, Thibault Viennet^{12

13}, Peter Tompa^{22

26

27}, Wim Vranken^{10

11

27}, Silvio C E Tosatto³, Norman E Davey²⁸

Affiliations

¹ Structural and Computational Biology Unit, European Molecular Biology Laboratory, Heidelberg, Germany.
² Department of Structural Biology and Center for Data Driven Discovery, St Jude Children's Research Hospital, Memphis, TN, USA.
³ Department of Biomedical Sciences, University of Padova, Padova, Italy.
⁴ Department of Oncology, Lausanne University Hospital, Lausanne, Switzerland.
⁵ Department of Computational Biology, University of Lausanne, Lausanne, Switzerland.
⁶ Swiss Institute of Bioinformatics, Lausanne, Switzerland.
⁷ Swiss Cancer Center Leman, Lausanne, Switzerland.
⁸ Departamento de Ciencia y Tecnología, Universidad Nacional de Quilmes - CONICET, Bernal, Buenos Aires, Argentina.
⁹ Institute of Biomembranes, Bioenergetics and Molecular Biotechnologies, National Research Council (CNR-IBIOM), Bari, Italy.
¹⁰ Interuniversity Institute of Bioinformatics in Brussels, ULB-VUB, Brussels, Belgium.
¹¹ Structural Biology Brussels, Vrije Universiteit Brussel, Brussels, Belgium.
¹² Harvard Medical School (HMS), Boston, MA, USA.
¹³ Department of Cancer Biology, Dana-Farber Cancer Institute (DFCI), Boston, MA, USA.
¹⁴ Department of Biochemistry, Eötvös Loránd University, Budapest, Hungary.
¹⁵ Department of Chemistry 'Ugo Schiff' and Magnetic Resonance Center, University of Florence, Sesto Fiorentino (Florence), Italy.
¹⁶ Department of Molecular Biology and Biophysics, UConn Health, Farmington, CT, USA.
¹⁷ European Molecular Biology Laboratory (EMBL), Hamburg Unit, c/o Deutsches Elektronen-Synchrotron, Hamburg, Germany.
¹⁸ Laboratory Architecture et Fonction des Macromolécules Biologiques (AFMB), UMR 7257, Aix Marseille University and Centre National de la Recherche Scientifique (CNRS), Marseille, France.
¹⁹ Cancer Structural Biology, Danish Cancer Society Research Center, Copenhagen, Denmark.
²⁰ UniCamillus - Saint Camillus International University of Health and Medical Sciences, Rome, Italy.
²¹ European Bioinformatics Institute (EMBL-EBI), European Molecular Biology Laboratory, Hinxton, UK.
²² Institute of Enzymology, Research Centre for Natural Sciences, Budapest, Hungary.
²³ Cancer Systems Biology, Section for Bioinformatics, Department of Health and Technology, Technical University of Denmark, Lyngby, Denmark.
²⁴ Hospital for Sick Children, Toronto, Ontario, Canada.
²⁵ Medical University of Graz, Graz, Austria.
²⁶ VIB-VUB Center for Structural Biology, Brussels, Belgium.
²⁷ Structural Biology Brussels, Department of Bioengineering Sciences, Vrije Universiteit Brussel, Brussels, Belgium.
²⁸ Division Of Cancer Biology, Institute of Cancer Research, Chester Beatty Laboratories, Chelsea, London, UK. norman.davey@icr.ac.uk.

^# Contributed equally.

PMID: 37400558
DOI: 10.1038/s41592-023-01915-x

Review

Minimum information guidelines for experiments structurally characterizing intrinsically disordered protein regions

Bálint Mészáros et al. Nat Methods. 2023 Sep.

. 2023 Sep;20(9):1291-1303.

doi: 10.1038/s41592-023-01915-x. Epub 2023 Jul 3.

Authors

Affiliations

¹ Structural and Computational Biology Unit, European Molecular Biology Laboratory, Heidelberg, Germany.
² Department of Structural Biology and Center for Data Driven Discovery, St Jude Children's Research Hospital, Memphis, TN, USA.
³ Department of Biomedical Sciences, University of Padova, Padova, Italy.
⁴ Department of Oncology, Lausanne University Hospital, Lausanne, Switzerland.
⁵ Department of Computational Biology, University of Lausanne, Lausanne, Switzerland.
⁶ Swiss Institute of Bioinformatics, Lausanne, Switzerland.
⁷ Swiss Cancer Center Leman, Lausanne, Switzerland.
⁸ Departamento de Ciencia y Tecnología, Universidad Nacional de Quilmes - CONICET, Bernal, Buenos Aires, Argentina.
⁹ Institute of Biomembranes, Bioenergetics and Molecular Biotechnologies, National Research Council (CNR-IBIOM), Bari, Italy.
¹⁰ Interuniversity Institute of Bioinformatics in Brussels, ULB-VUB, Brussels, Belgium.
¹¹ Structural Biology Brussels, Vrije Universiteit Brussel, Brussels, Belgium.
¹² Harvard Medical School (HMS), Boston, MA, USA.
¹³ Department of Cancer Biology, Dana-Farber Cancer Institute (DFCI), Boston, MA, USA.
¹⁴ Department of Biochemistry, Eötvös Loránd University, Budapest, Hungary.
¹⁵ Department of Chemistry 'Ugo Schiff' and Magnetic Resonance Center, University of Florence, Sesto Fiorentino (Florence), Italy.
¹⁶ Department of Molecular Biology and Biophysics, UConn Health, Farmington, CT, USA.
¹⁷ European Molecular Biology Laboratory (EMBL), Hamburg Unit, c/o Deutsches Elektronen-Synchrotron, Hamburg, Germany.
¹⁸ Laboratory Architecture et Fonction des Macromolécules Biologiques (AFMB), UMR 7257, Aix Marseille University and Centre National de la Recherche Scientifique (CNRS), Marseille, France.
¹⁹ Cancer Structural Biology, Danish Cancer Society Research Center, Copenhagen, Denmark.
²⁰ UniCamillus - Saint Camillus International University of Health and Medical Sciences, Rome, Italy.
²¹ European Bioinformatics Institute (EMBL-EBI), European Molecular Biology Laboratory, Hinxton, UK.
²² Institute of Enzymology, Research Centre for Natural Sciences, Budapest, Hungary.
²³ Cancer Systems Biology, Section for Bioinformatics, Department of Health and Technology, Technical University of Denmark, Lyngby, Denmark.
²⁴ Hospital for Sick Children, Toronto, Ontario, Canada.
²⁵ Medical University of Graz, Graz, Austria.
²⁶ VIB-VUB Center for Structural Biology, Brussels, Belgium.
²⁷ Structural Biology Brussels, Department of Bioengineering Sciences, Vrije Universiteit Brussel, Brussels, Belgium.
²⁸ Division Of Cancer Biology, Institute of Cancer Research, Chester Beatty Laboratories, Chelsea, London, UK. norman.davey@icr.ac.uk.

^# Contributed equally.

PMID: 37400558
DOI: 10.1038/s41592-023-01915-x

Abstract

An unambiguous description of an experiment, and the subsequent biological observation, is vital for accurate data interpretation. Minimum information guidelines define the fundamental complement of data that can support an unambiguous conclusion based on experimental observations. We present the Minimum Information About Disorder Experiments (MIADE) guidelines to define the parameters required for the wider scientific community to understand the findings of an experiment studying the structural properties of intrinsically disordered regions (IDRs). MIADE guidelines provide recommendations for data producers to describe the results of their experiments at source, for curators to annotate experimental data to community resources and for database developers maintaining community resources to disseminate the data. The MIADE guidelines will improve the interpretability of experimental results for data consumers, facilitate direct data submission, simplify data curation, improve data exchange among repositories and standardize the dissemination of the key metadata on an IDR experiment by IDR data sources.

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References

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Minimum information guidelines for experiments structurally characterizing intrinsically disordered protein regions

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Minimum information guidelines for experiments structurally characterizing intrinsically disordered protein regions

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