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. 1986 Jul;156(1):220-2.
doi: 10.1016/0003-2697(86)90176-4.

Enhancement by N-hydroxysulfosuccinimide of water-soluble carbodiimide-mediated coupling reactions

J V Staros, R W Wright, D M Swingle

Enhancement by N-hydroxysulfosuccinimide of water-soluble carbodiimide-mediated coupling reactions

J V Staros et al. Anal Biochem. 1986 Jul.

Abstract

Water-soluble carbodiimides are frequently employed in coupling or conjugation reactions, e.g., to link a peptide immunogen to a carrier protein. However, their utility is limited by low coupling yields obtained under some conditions. We have found that addition of N-hydroxysulfosuccinimide to such reactions can greatly enhance the yields obtained.

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