S haplotype collection in Brassicaceae crops-an updated list of S haplotypes

Abstract

Self-incompatibility is the system that inhibits pollen germination and pollen tube growth by self-pollen. This trait is important for the breeding of Brassica and Raphanus species. In these species, self-incompatibility is governed by the S locus, which contains three linked genes (a set called the S haplotype), i.e., S-locus receptor kinase, S-locus cysteine-rich protein/S-locus protein 11, and S-locus glycoprotein. A large number of S haplotypes have been identified in Brassica oleracea, B. rapa, and Raphanus sativus to date, and the nucleotide sequences of their many alleles have also been registered. In this state, it is important to avoid confusion between S haplotypes, i.e., an identical S haplotype with different names and a different S haplotype with an identical S haplotype number. To mitigate this issue, we herein constructed a list of S haplotypes that are easily accessible to the latest nucleotide sequences of S-haplotype genes, together with revisions to and an update of S haplotype information. Furthermore, the histories of the S-haplotype collection in the three species are reviewed, the importance of the collection of S haplotypes as a genetic resource is discussed, and the management of information on S haplotypes is proposed.

Keywords: Brassica; Raphanus; S haplotype; S-locus cysteine-rich protein/S-locus protein; S-locus glycoprotein; S-locus receptor kinase; self-incompatibility.

Fig. 1.

Linkage of SRK, SCR/SP11 and SLG at the S locus. Three genes, SRK, SCR/SP11 and SLG, located on the S locus and a set of alleles of three genes are called S haplotype.