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. 2023:2681:113-129.
doi: 10.1007/978-1-0716-3279-6_8.

Isolation of Antigen-Specific Unconventional Bovine Ultra-Long CDR3H Antibodies Using Cattle Immunization in Combination with Yeast Surface Display

Paul Arras  1 , Jasmin Zimmermann  1   2 , Britta Lipinski  1   2 , Desislava Yanakieva  1 , Daniel Klewinghaus  1 , Simon Krah  1 , Harald Kolmar  2 , Lukas Pekar  1 , Stefan Zielonka  3   4
Affiliations

Isolation of Antigen-Specific Unconventional Bovine Ultra-Long CDR3H Antibodies Using Cattle Immunization in Combination with Yeast Surface Display

Paul Arras et al. Methods Mol Biol. 2023.

Abstract

Cattle are known for their repertoire of antibodies harboring extremely long CDR3H regions that form extensive "knob on stalk" cysteine-rich structures. The compact knob domain allows for the recognition of epitopes potentially not accessible to classical antibodies. To effectively access the potential of bovine-derived antigen-specific ultra-long CDR3 antibodies, a straightforward and effective high-throughput method based on yeast surface display and fluorescence-activated cell sorting is described.

Keywords: Antibody display; Antibody engineering; Bovine ultra-long CDR3H antibodies; Stalk-knob; Yeast surface display.

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