Major Histocompatibility Complex-B haplotype and ovarian graft response

Abstract

Gonadal tissue transfer is considered one of the best methods to preserve genetic variability. Poultry hosts can receive a gonad from a donor of a different genetic background, sustain the growth of this graft, and produce gametes from it. Unfortunately, the host's strong immune response may significantly reduce the gonadal graft's ability to reach maturity. Our study aimed to evaluate the influence of MHC-B alleles in rejecting a gonadal graft of similar or different genetic backgrounds. In the first experiment, ovarian tissue was transplanted to chicks of similar genetic backgrounds, either Lohmann White (LW) with variable MHC-B or Barred Rock (BR) with fixed MHC-B. The sustained growth of donor ovarian tissues occurred in (4/7 hosts) BR (MHC-B matched) hosts only-one of these graft-positive-BR hens produced eggs derived from the donor ovary. No grafts were recovered when the host and the donor had an LW background (0/9; MHC-B mismatched). In the second experiment, ovarian transplantation was done between chicks of either similar or different genetic backgrounds (Brown Leghorn [BL], BR, and BL/BR F1). The 2 pure lines contained only one MHC-B allele, whereas the F1 heterozygotes had both. All host birds were given a daily dose of an immunosuppressant (mycophenolate mofetil) until maturity. The success rate was assessed by microsatellite genotype confirmation of donor-derived ovaries plus physiological and histological analyses of ovarian grafts. In this second experiment, 11 out of 43 ovarian hosts laid eggs. However, all fertilized eggs from these hens were derived from the remnant host ovarian tissue, not from the donor ovaries. A necropsy assessment was done on all 43 host birds. Ten donor grafts were recovered from hosts having matched (6 hosts) and mismatched (4 hosts) MHC-B, and none were functional. Interestingly, 6 of them were enclosed by a serous membrane capsule filled with fluid and had various tissue growth. In addition, clusters of immune cells were observed in all recovered donor grafts. Our results demonstrated that genetic background could greatly influence the success of gonadal transfer in chickens.

Keywords: MHC-B; gonadal transfer; immunosuppression; ovary; vitrification.

Figure 1

Necropsy assessment of adult hens. Grafts were covered with membranes filled with fluid (A–D) or located at the bottom of the host's ovaries (E, F) showed inside the opened abdomen cavity and detached membranes with fluid (bottom corner of pictures). The size and shape of ovarian tissues varied from hen to hen (ranging from 0.5 to 4 cm).

Figure 2

H&E-stained ovarian grafts. H&E-stained ovarian grafts showed a sign of inflammatory response with macrophages (M), lymphocytes (L), and heterophils (H) at the left corner with arrows (1,000× magnification) in images (B), (C), and (D), respectively at 400× magnification. Normal histological morphology of the control ovary showed in image (A) at 200× magnification.