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. 2023 Dec 1;107(12):2568-2574.
doi: 10.1097/TP.0000000000004712. Epub 2023 Jul 6.

Urine CXCL10 to Assess BK Polyomavirus Replication After Kidney Transplantation

Jana Haller  1 , Matthias Diebold  1 , Karoline Leuzinger  2   3 , Caroline Wehmeier  1 , Joelle Handschin  1   4 , Patrizia Amico  1 , Patricia Hirt-Minkowski  1 , Jürg Steiger  1 , Michael Dickenmann  1 , Hans H Hirsch  2   3 , Stefan Schaub  1   4   5
Affiliations

Urine CXCL10 to Assess BK Polyomavirus Replication After Kidney Transplantation

Jana Haller et al. Transplantation. .

Abstract

Background: Urine CXCL10 is a biomarker for renal allograft inflammation induced by rejection, urinary tract infection, or BK polyomavirus (BKPyV) replication. This study aimed to compare urine CXCL10 levels in different stages of BKPyV reactivation and to investigate urine CXCL10 as a biomarker for BKPyV replication.

Methods: We included 763 urine samples (235 patients) from an interventional, randomized trial obtained in the context of regular screening for urine CXCL10 levels. All urine samples had a complete urine sediment analysis, no rejection episode noted within 30 d before urine collection, and a urine decoy cell analysis was conducted within ±3 d.

Results: Urine CXCL10 levels were 2.31 ng/mmol in samples without BKPyV viruria, slightly rose to 4.35 ng/mmol with BKPyV viruria, and then markedly increased to 16.42 ng/mmol when decoy cells were detectable, but still in the absence of BKPyV DNAemia ( P < 0.001). The highest urine CXCL10 values were observed in samples with BKPyV DNAemia (median 42.59 ng/mmol). The area under the curve of urine CXCL10 levels to detect ≥3 decoy cells was 0.816. At a CXCL10 cutoff of 3 ng/mmol, the negative predictive value was 97%. The area under the curve of urine CXCL10 levels to detect BKPyV DNAemia was 0.882, with a negative predictive value of 99% at a CXCL10 cutoff of 3 ng/mmol.

Conclusions: Urine CXCL10 levels are already significantly elevated in BKPyV viruria (especially with decoy cell shedding) and further increase with BKPyV DNAemia. Low urine CXCL10 values can rule out the presence of ≥3 decoy cells and BKPyV DNAemia with high certainty.

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Conflict of interest statement

The authors declare no conflicts of interest.

Figures

None
Graphical abstract
FIGURE 1.
FIGURE 1.
Flowchart. BKPyV, BK polyomavirus; HPF, high-power field.
FIGURE 2.
FIGURE 2.
Urine CXCL10 in different stages of BKPyV reactivation. BKPyV, BK polyomavirus.
FIGURE 3.
FIGURE 3.
Urine CXCL10 in different phases of patients with BKPyV DNAemia. BKPyV, BK polyomavirus.
FIGURE 4.
FIGURE 4.
Diagnostic performance of urine CXCL10 for detection of BKPyV replication. A, Detection of ≥3 decoy cells. B, Detection of BKPyV DNAemia. AUC, area under the curve; BKPyV, BK polyomavirus; NPV, negative predictive value; PPV, positive predictive value.
See this image and copyright information in PMC

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