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. 2023:2688:71-82.
doi: 10.1007/978-1-0716-3319-9_7.

The Adaptation of the QV600 LLI Milli-Fluidics System to House Ex Vivo Gastrointestinal Tissue Suitable for Drug Absorption and Permeation Studies, Utilizing MALDI MSI and LC-MS/MS

Chloe E Spencer  1 , Catherine J Duckett  1 , Stephen Rumbelow  2 , Malcolm R Clench  3
Affiliations

The Adaptation of the QV600 LLI Milli-Fluidics System to House Ex Vivo Gastrointestinal Tissue Suitable for Drug Absorption and Permeation Studies, Utilizing MALDI MSI and LC-MS/MS

Chloe E Spencer et al. Methods Mol Biol. 2023.

Abstract

Careful formulation of pharmaceuticals for oral delivery is essential to ensure that the optimal amount of the active ingredient reaches its intended site of action. This chapter demonstrates how mass spectrometry can be used in conjunction with ex vivo tissue and an adapted milli-fluidics system to carry out a drug absorption study. MALDI MSI is used to visualize the drug within the small intestine tissue from the absorption experimentation. LC-MS/MS is used to complete a mass balance of the experiment and quantify the amount of drug that has permeated through the tissue.

Keywords: Gastrointestinal; Milli-fluidics system; Pharmaceuticals; Transendothelial electrical resistance; Transepithelial.

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