Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1986 Apr 1;235(1):177-82.
doi: 10.1042/bj2350177.

Streptomyces K15 DD-peptidase-catalysed reactions with suicide beta-lactam carbonyl donors

M Leyh-Bouille, M Nguyen-Distèche, S Pirlot, A Veithen, C Bourguignon, J M Ghuysen

Streptomyces K15 DD-peptidase-catalysed reactions with suicide beta-lactam carbonyl donors

M Leyh-Bouille et al. Biochem J. .

Erratum in

  • Biochem J 1986 Jun 15;236(3):following 933

Abstract

The values of the kinetic parameters that govern the interactions between the Streptomyces K15 DD-peptidase and beta-lactam compounds were determined by measuring the inactivating effect that these compounds exert on the transpeptidase activity of the enzyme and, in the case of [14C]benzylpenicillin and [14C]cefoxitin, by measuring the amounts of acyl-enzyme formed during the reaction. K15 DD-peptidase binds benzylpenicillin or cefoxitin at a molar ratio of 1:1. Benzylpenicilloate is the major product released during breakdown of the acyl-enzyme formed with benzylpenicillin. Benzylpenicillin is not a better acylating agent than the amide Ac2-L-Lys-D-Ala-D-Ala and ester Ac2-L-Lys-D-Ala-D-lactatecarbonyl-donor substrates. beta-Lactam compounds possessing a methoxy group on the alpha-face of the molecule show high inactivating potency.

PubMed Disclaimer

References

    1. J Mol Biol. 1973 Nov 15;80(4):575-99 - PubMed
    1. Eur J Biochem. 1974 Dec 16;50(1):203-14 - PubMed
    1. Methods Enzymol. 1975;43:678-87 - PubMed
    1. Methods Enzymol. 1975;43:687-98 - PubMed
    1. Eur J Biochem. 1977 Nov 15;81(1):19-28 - PubMed

Publication types

MeSH terms