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. 1986 Apr 1;235(1):301-3.
doi: 10.1042/bj2350301.

Purification and characterization of dihydrofolate reductase from Mycobacterium phlei

M Al-Rubeai, J W Dale

Purification and characterization of dihydrofolate reductase from Mycobacterium phlei

M Al-Rubeai et al. Biochem J. .

Abstract

The dihydrofolate reductase from Mycobacterium phlei was purified and characterized; it has an Mr of 15 000 and a pI of 4.8. It is competitively inhibited by both methotrexate and trimethoprim, although the affinity is less than for other bacterial dihydrofolate reductases.

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