doi: 10.1016/s0006-291x(86)80561-7.
A high melting cis-[Pt(NH3)2[d(GpG)]]adduct of a decanucleotide duplex
- PMID: 3741432
- DOI: 10.1016/s0006-291x(86)80561-7
Item in Clipboard
A high melting cis-[Pt(NH3)2[d(GpG)]]adduct of a decanucleotide duplex
Biochem Biophys Res Commun.
.
Abstract
The [cis-Pt(NH3)2(d(GCCGGATCGC)-N7(4), N7(5))]-d(GCGATCCGGC) duplex has been prepared with Tm = 49 degrees C (vs 58 degrees C for the unplatinated form). NMR of the ten observable imino protons supports a kinked structure with intact base pairing of the duplex on the 3'-side of the d(GpG).cis-Pt chelate (relative to the platinated strand) The modification of the B-DNA type CD spectrum, due to the platinum chelate, is comparable to that observed for the platination (at a 0.05 Pt:base ratio) of the Micrococcus Lysodeikticus DNA (72% GC).
Similar articles
-
Synthesis and characterization of a d(ApG) platinated nonanucleotide duplex.Biochem Biophys Res Commun. 1992 Jan 31;182(2):555-60. doi: 10.1016/0006-291x(92)91768-l. Biochem Biophys Res Commun. 1992. PMID: 1734870
-
Chemical and biological studies of the major DNA adduct of cis-diamminedichloroplatinum(II), cis-[Pt(NH3)2(d(GpG]], built into a specific site in a viral genome.Biochemistry. 1988 Jun 14;27(12):4357-67. doi: 10.1021/bi00412a024. Biochemistry. 1988. PMID: 3166983
-
A d(GpG)-platinated decanucleotide duplex is kinked. An extended NMR and molecular mechanics study.Eur J Biochem. 1990 Nov 26;194(1):119-33. doi: 10.1111/j.1432-1033.1990.tb19435.x. Eur J Biochem. 1990. PMID: 2174772
-
Formation and structure of reaction products of cis-PtCl2(NH3)2 with d(ApG) and/or d(GpA) in di-, tri- and penta-nucleotides. Preference for GpA chelation over ApG chelation.Eur J Biochem. 1989 Feb 1;179(2):335-44. doi: 10.1111/j.1432-1033.1989.tb14559.x. Eur J Biochem. 1989. PMID: 2537207
-
Monofunctional platinum amine complexes destabilize DNA significantly.Eur J Biochem. 1998 Sep 1;256(2):253-60. doi: 10.1046/j.1432-1327.1998.2560253.x. Eur J Biochem. 1998. PMID: 9760162
Cited by
-
Spectrum of cisplatin-induced mutations in Escherichia coli.Proc Natl Acad Sci U S A. 1987 Jun;84(11):3758-62. doi: 10.1073/pnas.84.11.3758. Proc Natl Acad Sci U S A. 1987. PMID: 3295870 Free PMC article.
-
Influence of cisplatin intrastrand crosslinking on the conformation, thermal stability, and energetics of a 20-mer DNA duplex.Proc Natl Acad Sci U S A. 1996 Jul 23;93(15):7606-11. doi: 10.1073/pnas.93.15.7606. Proc Natl Acad Sci U S A. 1996. PMID: 8755522 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Miscellaneous
