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. 1986 Jul 31;138(2):758-63.
doi: 10.1016/s0006-291x(86)80561-7.

A high melting cis-[Pt(NH3)2[d(GpG)]]adduct of a decanucleotide duplex

B van Hemelryck, E Guittet, G Chottard, J P Girault, F Herman, T Huynh-Dinh, J Y Lallemand, J Igolen, J C Chottard

A high melting cis-[Pt(NH3)2[d(GpG)]]adduct of a decanucleotide duplex

B van Hemelryck et al. Biochem Biophys Res Commun. .

Abstract

The [cis-Pt(NH3)2(d(GCCGGATCGC)-N7(4), N7(5))]-d(GCGATCCGGC) duplex has been prepared with Tm = 49 degrees C (vs 58 degrees C for the unplatinated form). NMR of the ten observable imino protons supports a kinked structure with intact base pairing of the duplex on the 3'-side of the d(GpG).cis-Pt chelate (relative to the platinated strand) The modification of the B-DNA type CD spectrum, due to the platinum chelate, is comparable to that observed for the platination (at a 0.05 Pt:base ratio) of the Micrococcus Lysodeikticus DNA (72% GC).

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