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. 2023 Jan-Dec;15(1):2232506.
doi: 10.1080/19490976.2023.2232506.

Dynamic effects of probiotic formula ecologic®825 on human small intestinal ileostoma microbiota: a network theory approach

Jack Jansma  1 , Nicola U Thome  2 , Markus Schwalbe  1 , Anastasia Chrysovalantou Chatziioannou  1 , Somayah S Elsayed  2 , Gilles P van Wezel  2   3 , Pieter van den Abbeele  4 , Saskia van Hemert  5 , Sahar El Aidy  1
Affiliations

Dynamic effects of probiotic formula ecologic®825 on human small intestinal ileostoma microbiota: a network theory approach

Jack Jansma et al. Gut Microbes. 2023 Jan-Dec.

Abstract

The gut microbiota plays a pivotal role in health and disease. The use of probiotics as microbiota-targeted therapies is a promising strategy to improve host health. However, the molecular mechanisms involved in such therapies are often not well understood, particularly when targeting the small intestinal microbiota. In this study, we investigated the effects of a probiotic formula (Ecologic®825) on the adult human small intestinal ileostoma microbiota. The results showed that supplementation with the probiotic formula led to a reduction in the growth of pathobionts, such as Enterococcaceae and Enterobacteriaceae, and a decrease in ethanol production. These changes were associated with significant alterations in nutrient utilization and resistance to perturbations. These probiotic mediated alterations which coincided with an initial increase in lactate production and decrease in pH were followed by a sharp increase in the levels of butyrate and propionate. Moreover, the probiotic formula increased the production of multiple N-acyl amino acids in the stoma samples. The study demonstrates the utility of network theory in identifying novel microbiota-targeted therapies and improving existing ones. Overall, the findings provide insights into the dynamic molecular mechanisms underlying probiotic therapies, which can aid in the development of more effective treatments for a range of conditions.

Keywords: Probiotics; dynamic correlation-based networks; gut microbiota; metabolites; sequencing; small intestine.

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Conflict of interest statement

S. van Hemert is employee in Winclove (Winclove manufactures and markets probiotics). The content of this study was neither influenced nor constrained by this fact. The other authors have no conflicts of interest to declare.

Figures

Figure 1.
Figure 1.
A probiotic formula reduces the cell counts of Enterococcus and Klebsiella species in compositional data obtained from ileostomy effluent.
Figure 2.
Figure 2.
A probiotic formula alters the metabolic environment when supplemented to ileostomy effluent.
Figure 3.
Figure 3.
Dynamic correlations within the microbiota show a different dynamic metabolic fingerprint of the community with and without probiotic supplementation.
Figure 4.
Figure 4.
The probiotics supplementation to the ileostoma microbiota alters the metabolic environment and microbiota composition in a pH dependent manner.
See this image and copyright information in PMC

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