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. 2024:2767:293-305.
doi: 10.1007/7651_2023_494.

Manual Dissociation of Mammalian Preimplantation Embryos for Single-Cell Genomics

Katherine Vandal  1   2 , Savana Biondic  1   2 , Jesica Canizo  1   2 , Sophie Petropoulos  1   2   3
Affiliations

Manual Dissociation of Mammalian Preimplantation Embryos for Single-Cell Genomics

Katherine Vandal et al. Methods Mol Biol. 2024.

Abstract

Single-cell genomics allow the characterization and quantification of molecular heterogeneity from a wide variety of tissues. Here, we describe the manual dissociation and collection of single cells, a method adapted for the characterization of precious small tissues like preimplantation embryos. We also describe the acquisition of mouse embryos by flushing of the oviducts. The cells can then be used in multiple sequencing protocols, for example, Smart-seq2, Smart-seq3, smallseq, and scBSseq.

Keywords: Embryo collection; Human; Mouse; Preimplantation embryo; Sequencing; Single-cell.

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