Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1986;40(3):100-5.

Estimation by high-performance liquid chromatography of ketoprofen in plasma. Application to the study of its protein binding

R J Royer, F Lapicque, P Netter, C Monot, B Bannwarth, M C Cure
  • PMID: 3742007

Estimation by high-performance liquid chromatography of ketoprofen in plasma. Application to the study of its protein binding

R J Royer et al. Biomed Pharmacother. 1986.

Abstract

A method of estimation by high-performance liquid chromatography of ketoprofen in plasma is proposed. This method is rapid, simple, and very sensitive: it allows concentrations of 2 ng/ml to be determined. The internal standard is (benzoyl-4-phenyl)-2-butyric acid. The drug is extracted from a biological fluid in ether, which is then evaporated; the residue is taken up in chromatographic eluant (acetonitrile/phosphate buffer pH 7), analysed by reverse-phase chromatography and detected by spectrophotometry at 256 nm. In spite of the very strong protein binding of this drug, this method allowed the determination of its free concentration in plasma.

PubMed Disclaimer